Related papers: Adaptive Optics for Fluorescence Correlation Spect…
Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…
Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…
The point-spread function (PSF) of an adaptive optics (AO) system is often poorly known. This ignorance can lead to significant systematic errors in photometry. Since the degree of AO correction is sensitive to the observing conditions:…
Photon correlation spectroscopy (PCS) is based on measuring the temporal correlation of the light intensity scattered by the investigated sample. A typical setup requires a temporally coherent light source. Here, we show that a…
Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…
Single-molecule sensitive microscopies and spectroscopies are transforming biophysics and materials science laboratories. Techniques such as fluorescence correlation spectroscopy (FCS) and single-molecule sensitive fluorescence resonance…
Achieving atomic resolution in electron microscopy has historically been hindered by spherical aberration, a fundamental limitation of conventional electron lenses. Its correction typically requires complex assemblies of electromagnetic…
Direct imaging of exoplanets requires very high contrast levels, which are obtained using coronagraphs. But residual quasi-static aberrations create speckles in the focal plane downstream of the coronagraph which mask the planet. This…
Strong turbulence conditions create amplitude aberrations through the effects of near-field diffraction. When integrated over long optical path lengths, amplitude aberrations (seen as scintillation) can nullify local areas in the recorded…
Spectrographs nominally contain a degree of quasi-static optical aberrations resulting from the quality of manufactured component surfaces, imperfect alignment, design residuals, thermal effects, and other other associated phenomena…
Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…
In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. Based on a dynamic correction of the incident…
Accurate astrometry and photometry of saturated and coronagraphic point spread functions (PSFs) are fundamental to both ground- and space-based high contrast imaging projects. For ground-based adaptive optics imaging, differential…
An optical field will undergo coherent diffusion when it is mapped into thermal-motioned atoms, e.g., in a slow or storage light process. As was demonstrated before, such diffusion effect is equivalent to a spatial low-pass filter…
Optical beams and starlight distorted by atmospheric turbulence can be corrected with adaptive optics systems to enable efficient coupling into single-mode fibers. Deformable mirrors, used to flatten the wavefront in astronomical…
We present a new method that combines fluorescence correlation spectroscopy (FCS) on the microsecond time scale with fluorescence antibunching measurements on the nanosecond time scale for measuring photophysical rate constants of…
Telescope Point Spread Function (PSF) quality is critical for realising the potential of cosmic weak lensing observations to constrain dark energy and test General Relativity. In this paper we use quantitative weak gravitational lensing…
There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…
Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…
Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…