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Despite great interest in solving RNA secondary structures due to their impact on function, it remains an open problem to determine structure from sequence. Among experimental approaches, a promising candidate is the "chemical modification…

Quantitative Methods · Quantitative Biology 2011-06-30 Sharon Aviran , Julius B. Lucks , Lior Pachter

RNA function is tied to secondary structure, operating through dynamic and heterogeneous structural ensembles. While current analysis tools typically output single static structures or averaged contact maps, chemical probing methods like…

Biomolecules · Quantitative Biology 2026-05-20 Giuseppe Sacco , Jianhui Li , Redmond P. Smyth , Guido Sanguinetti , Giovanni Bussi

Structural prediction has long been considered critical in RNA research, especially following the success of AlphaFold2 in protein studies, which has drawn significant attention to the field. While recent advances in machine learning and…

Biomolecules · Quantitative Biology 2024-09-26 Jiaxing Yang

Non-coding RNA molecules fold into precise base pairing patterns to carry out critical roles in genetic regulation and protein synthesis. We show here that coupling systematic mutagenesis with high-throughput SHAPE chemical mapping enables…

Quantitative Methods · Quantitative Biology 2011-04-07 Wipapat Kladwang , Christopher C. VanLang , Pablo Cordero , Rhiju Das

Research resources (RRs) such as data, software, and tools are essential pillars of scientific research. The field of biomedicine, a critical scientific discipline, is witnessing a surge in research publications resulting in the…

Digital Libraries · Computer Science 2024-09-24 Li Zhang , Mengting Sun , Chong Jiang , Haihua Chen

For decades, dimethyl sulfate (DMS) mapping has informed manual modeling of RNA structure in vitro and in vivo. Here, we incorporate DMS data into automated secondary structure inference using a pseudo-energy framework developed for 2'-OH…

Biomolecules · Quantitative Biology 2012-07-06 Pablo Cordero , Wipapat Kladwang , Christopher C. VanLang , Rhiju Das

RNAnet provides a bridge between two widely used Human gene databases. Ensembl describes DNA sequences and transcripts but not experimental gene expression. Whilst NCBI's GEO contains actual expression levels from Human samples. RNAnet…

Molecular Networks · Quantitative Biology 2010-01-26 W. B. Langdon , Olivia Sanchez Graillet , A. P. Harrison

Relational databases (RDBs) have become the industry standard for storing massive and heterogeneous data. However, despite the widespread use of RDBs across various fields, the inherent structure of relational databases hinders their…

Databases · Computer Science 2025-08-13 Ning Li , Kounianhua Du , Han Zhang , Quan Gan , Minjie Wang , David Wipf , Weinan Zhang

Chemical mapping methods probe RNA structure by revealing and leveraging correlations of a nucleotide's structural accessibility or flexibility with its reactivity to various chemical probes. Pioneering work by Lucks and colleagues has…

Biomolecules · Quantitative Biology 2013-04-04 Matthew G. Seetin , Wipapat Kladwang , J. P. Bida , Rhiju Das

Summary: In anticipation of the individualized proteomics era and the need to integrate knowledge from disease studies, we have augmented our peptide identification software RAId DbS to take into account annotated single amino acid…

Quantitative Methods · Quantitative Biology 2008-03-18 Gelio Alves , Aleksey Ogurtsov , Yi-Kuo Yu

Chemical mapping is a widespread technique for structural analysis of nucleic acids in which a molecule's reactivity to different probes is quantified at single-nucleotide resolution and used to constrain structural modeling. This…

Biomolecules · Quantitative Biology 2013-02-01 Pablo Cordero , Wipapat Kladwang , Christopher C. VanLang , Rhiju Das

Benefiting from high-quality datasets and standardized evaluation metrics, machine learning (ML) has achieved sustained progress and widespread applications. However, while applying machine learning to relational databases (RDBs), the…

Machine Learning · Computer Science 2023-10-31 Zizhao Zhang , Yi Yang , Lutong Zou , He Wen , Tao Feng , Jiaxuan You

Accurate RNA structure modeling remains difficult because RNA backbones are highly flexible, non-canonical interactions are prevalent, and experimentally determined 3D structures are comparatively scarce. We introduce \emph{RiboSphere}, a…

Machine Learning · Computer Science 2026-03-23 Zhou Zhang , Hanqun Cao , Cheng Tan , Fang Wu , Pheng Ann Heng , Tianfan Fu

The intricate network of interactions observed in RNA three-dimensional structures is often described in terms of a multitude of geometrical properties, including helical parameters, base pairing/stacking, hydrogen bonding and backbone…

Biomolecules · Quantitative Biology 2015-09-01 Sandro Bottaro , Francesco Di Palma , Giovanni Bussi

Single-cell RNA sequencing (scRNA-seq) is a relatively new technology that has stimulated enormous interest in statistics, data science, and computational biology due to the high dimensionality, complexity, and large scale associated with…

Machine Learning · Statistics 2023-10-25 Yuta Hozumi , Guo-Wei Wei

While artificial intelligence has made remarkable strides in revealing the relationship between biological macromolecules' primary sequence and tertiary structure, designing RNA sequences based on specified tertiary structures remains…

Biomolecules · Quantitative Biology 2024-03-08 Cheng Tan , Yijie Zhang , Zhangyang Gao , Bozhen Hu , Siyuan Li , Zicheng Liu , Stan Z. Li

The Nearest Neighbor model is the $\textit{de facto}$ thermodynamic model of RNA secondary structure formation and is a cornerstone of RNA structure prediction and sequence design. The current functional form (Turner 2004) contains…

Biomolecules · Quantitative Biology 2025-05-13 Ryan K. Krueger , Sharon Aviran , David H. Mathews , Jeffrey Zuber , Max Ward

Large RNA molecules often carry multiple functional domains whose spatial arrangement is an important determinant of their function. Pre-mRNA splicing, furthermore, relies on the spatial proximity of the splice junctions that can be…

Quantitative Methods · Quantitative Biology 2013-07-31 Rolf Backofen , Markus Fricke , Manja Marz , Jing Qin , Peter F. Stadler

Metabolite structure identification has become the major bottleneck of the mass spectrometry based metabolomics research. Till now, number of mass spectra databases and search algorithms have been developed to address this issue. However,…

Quantitative Methods · Quantitative Biology 2021-01-12 Hansen Zhao , Xu Zhao , Huan Yao , Jiaxin Feng , Sichun Zhang , Xinrong Zhang

Messenger RNA-based medicines hold immense potential, as evidenced by their rapid deployment as COVID-19 vaccines. However, worldwide distribution of mRNA molecules has been limited by their thermostability, which is fundamentally limited…

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