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Fluorescence microscopy reveals that the contents of many (membrane-free) nuclear "bodies" exchange rapidly with the soluble pool whilst the underlying structure persists; such observations await a satisfactory biophysical explanation. To…

Biological Physics · Physics 2017-04-26 C. A. Brackley , B. Liebchen , D. Michieletto , F. Mouvet , P. R. Cook , D. Marenduzzo

Prediction of protein-ligand binding affinity is a major goal in drug discovery. Generally, free energy gap is calculated between two states (e.g., ligand binding and unbinding). The energy gap implicitly includes the effects of changes in…

Biomolecules · Quantitative Biology 2022-05-20 Ikki Yasuda , Katsuhiro Endo , Eiji Yamamoto , Yoshinori Hirano , Kenji Yasuoka

In this paper, a new method to efficiently simulate diffusion controlled second order chemical reactions is derived and applied to site-specific DNA-binding proteins. The protein enters a spherical cell and propagates via two competing…

Biological Physics · Physics 2009-11-11 Holger Merlitz , Konstantin V. Klenin , Chen-Xu Wu , Jörg Langowski

Recent advances in operating and manipulating DNA have provided unique experimental possibilities in many fields of DNA research, especially in gene therapy. Researchers have deployed many techniques, experimental and theoretical, to study…

Soft Condensed Matter · Physics 2023-01-04 Arghya Maity , Neha Mathur , Petra Imhof , Navin Singh

Single molecule and NMR measurements of protein dynamics increasingly uncover the complexity of binding scenarios. Here we describe an extended conformational selection model which embraces a repertoire of selection and adjustment…

Biomolecules · Quantitative Biology 2010-10-05 Peter Csermely , Robin Palotai , Ruth Nussinov

Protein motions occur on multiple time and distance scales. Large-scale motions of protein tertiary-structure elements, i.e. domains, are particularly intriguing as they are essential for the catalytic activity of many enzymes and for the…

Biological Physics · Physics 2020-10-28 Gilad Haran , Hisham Mazal

Deep neural network (DNN) based approaches have been widely investigated and deployed in medical image analysis. For example, fully convolutional neural networks (FCN) achieve the state-of-the-art performance in several applications of…

Computer Vision and Pattern Recognition · Computer Science 2020-06-11 Dong Yang , Holger Roth , Ziyue Xu , Fausto Milletari , Ling Zhang , Daguang Xu

Proteins play crucial roles in every cellular process by interacting with each other, with nucleic acids, metabolites, and other molecules. The resulting assemblies can be very large and intricate and pose challenges to experimental…

Biomolecules · Quantitative Biology 2021-03-16 Charlotte W. van Noort , Rodrigo V. Honorato , Alexandre M. J. J. Bonvin

A dynamical model is presented for chiral change in DNA molecules. The model is an extension of the conventional elastic model which incorporates the structure of base pairs and uses a spinor representation for the DNA configuration…

Biological Physics · Physics 2011-10-06 Teruaki Okushima , Hiroshi Kuratsuji

Protein similarity searches are a routine job for molecular biologists where a query sequence of amino acids needs to be compared and ranked against an ever-growing database of proteins. All available algorithms in this field can be grouped…

Computational Engineering, Finance, and Science · Computer Science 2015-08-27 Akash Nag , Sunil Karforma

All known life forms are based upon a hierarchy of interwoven feedback loops, operating over a cascade of space, time and energy scales. Among the most basic loops are those connecting DNA and proteins. For example, in genetic networks, DNA…

Other Quantitative Biology · Quantitative Biology 2018-04-13 Tsvi Tlusty

Safe use of Deep Neural Networks (DNNs) requires careful testing. However, deployed models are often trained further to improve in performance. As rigorous testing and evaluation is expensive, triggers are in need to determine the degree of…

Machine Learning · Computer Science 2021-11-04 Konstantin Schürholt , Damian Borth

In recent years, machine learning has been proposed as a promising strategy to build accurate scoring functions for computational docking finalized to numerically empowered drug discovery. However, the latest studies have suggested that…

Quantitative Methods · Quantitative Biology 2023-02-17 F. Pellicani , D. Dal Ben , A. Perali , S. Pilati

To sample rare events, dissipation-corrected targeted molecular dynamics (dcTMD) applies a constant velocity constraint along a one-dimensional reaction coordinate $s$, which drives an atomistic system from an initial state into a target…

Biological Physics · Physics 2023-09-19 Matthias Post , Steffen Wolf , Gerhard Stock

A fundamental goal of research in molecular biology is to understand protein structure. Protein crystallography is currently the most successful method for determining the three-dimensional (3D) conformation of a protein, yet it remains…

Artificial Intelligence · Computer Science 2014-11-17 L. Leherte , J. Glasgow , K. Baxter , E. Steeg , S. Fortier

The structure of molecular networks derives from dynamical processes on evolutionary time scales. For protein interaction networks, global statistical features of their structure can now be inferred consistently from several…

Statistical Mechanics · Physics 2007-05-23 Johannes Berg , Michael Lässig , Andreas Wagner

We study numerically the mechanical stability and elasticity properties of duplex DNA molecules within the frame of a network model incorporating microscopic degrees of freedom related with the arrangement of the base pairs. We pay special…

Pattern Formation and Solitons · Physics 2015-06-26 D. Hennig , J. F. R. Archilla

Self-avoiding random walks were performed on protein residue networks. Compared with protein residue networks with randomized links, the probability of a walk being successful is lower and the length of successful walks shorter in…

Molecular Networks · Quantitative Biology 2013-06-11 Susan Khor

A multiscale approach is used to simulate the translocation of DNA through a nanopore. Within this scheme, the interactions of the molecule with the surrounding fluid (solvent) are explicitly taken into account. By generating polymers of…

Biological Physics · Physics 2009-11-13 Simone Melchionna , Maria Fyta , Efthimios Kaxiras , Sauro Succi

In many biochemical processes, proteins bound to DNA at distant sites are brought into close proximity by loops in the underlying DNA. For example, the function of some gene-regulatory proteins depends on such DNA looping interactions. We…

Quantitative Methods · Quantitative Biology 2009-11-13 John F Beausang , Philip C Nelson