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In this work, an improved methodology for studying interactions of proteins in solution by small-angle scattering, is presented. Unlike the most common approach, where the protein-protein correlation functions $g_{ij}(r)$ are approximated…

Statistical Mechanics · Physics 2009-11-07 Francesco Spinozzi , Domenico Gazzillo , Achille Giacometti , Paolo Mariani , Flavio Carsughi

We describe a combination of all-atom simulations with CABS, a well-established coarse-grained protein modeling tool, into a single multiscale protocol. The simulation method has been tested on the C-terminal beta hairpin of protein G, a…

Biological Physics · Physics 2013-08-13 Jacek Wabik , Sebastian Kmiecik , Dominik Gront , Maksim Kouza , Andrzej Kolinski

In this paper we report a fiber optical sensor system based on surface plasmon resonance (SPR) with real-time response for biochemical interaction analysis. The fiber sensor is constructed from a multi-mode fiber with plastic cladding. To…

Applied Physics · Physics 2019-05-10 Zhixin Tan , Xuejin Li , Yuzhi Chen , Ping Fan

Large numbers of MS/MS peptide spectra generated in proteomics experiments require efficient, sensitive and specific algorithms for peptide identification. In the Open Mass Spectrometry Search Algorithm [OMSSA], specificity is calculated by…

Quantitative Methods · Quantitative Biology 2007-05-23 Lewis Y. Geer , Sanford P. Markey , Jeffrey A. Kowalak , Lukas Wagner , Ming Xu , Dawn M. Maynard , Xiaoyu Yang , Wenyao Shi , Stephen H. Bryant

In this work, we report a 5-minute magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface functionalized magnetic nanoparticles (MNPs) are incubated with target analytes at 37 {\deg}C with agitation for 3…

Approximate Bayesian computation (ABC) is an approach for sampling from an approximate posterior distribution in the presence of a computationally intractable likelihood function. A common implementation is based on simulating model,…

Methodology · Statistics 2013-01-16 D. Prangle , M. G. B. Blum , G. Popovic , S. A. Sisson

Doping control is an essential component of anti-doping organizations for protecting clean sports competitions. Since 2009, this mission has been complemented worldwide by the Athlete Biological Passport (ABP), used to monitor athletes'…

Methodology · Statistics 2024-04-22 Nina Deliu , Brunero Liseo

regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal…

Quantitative Methods · Quantitative Biology 2020-09-01 Aleksandra A. Petelski , Nikolai Slavov

Mammography is the primary imaging modality used for early detection and diagnosis of breast cancer. X-ray mammogram analysis mainly refers to the localization of suspicious regions of interest followed by segmentation, towards further…

Image and Video Processing · Electrical Eng. & Systems 2020-12-09 Yutong Yan , Pierre-Henri Conze , Gwenolé Quellec , Mathieu Lamard , Béatrice Cochener , Gouenou Coatrieux

Proteins play a pivotal role in biological systems. The use of machine learning algorithms for protein classification can assist and even guide biological experiments, offering crucial insights for biotechnological applications. We…

Quantitative Methods · Quantitative Biology 2024-10-24 Yizheng Wang , Yixiao Zhai , Yijie Ding , Quan Zou

We present a novel method to determine eccentricity constraints of extrasolar planets in systems with multiple transiting planets through photometry alone. Our method is highly model independent, making no assumptions about the stellar…

Earth and Planetary Astrophysics · Physics 2015-06-03 David M. Kipping , William R. Dunn , Jamie M. Jasinski , Varun P. Manthri

Tuberculosis (TB) is a contagious disease that causes 1.5 million deaths per year globally. Early diagnosis of TB patients is critical to control its spread. However, standard TB diagnostic tests such as sputum culture take days to weeks to…

Medical Physics · Physics 2022-08-26 Ubaid Ullah , Zarfishan Tahir , Obaidullah Qazi , Shaper Mirza , M. Imran Cheema

Proteomic matrix-assisted laser desorption/ionisation (MALDI) linear time-of-flight (TOF) mass spectrometry (MS) may be used to produce protein profiles from biological samples with the aim of discovering biomarkers for disease. However,…

Quantitative Methods · Quantitative Biology 2016-03-24 Tyman E. Stanford , Christopher J. Bagley , Patty J. Solomon

We present results from an instrument combining surface acoustic wave (SAW) propagation and surface plasmon resonance (SPR) measurements. The objective is to use two independent methods, the former based on adsorbed mass change measurements…

Soft Condensed Matter · Physics 2009-11-10 J. -M. Friedt , L. Francis , G. Reekmans , R. De Palma , A. Campitelli , U. B. Sleytr

Multivalent associative proteins with strong complementary interactions play a crucial role in phase separation of intracellular liquid condensates. We study the internal dynamics of such "bond-network" condensates comprised of two…

Soft Condensed Matter · Physics 2022-02-02 Pierre Ronceray , Yaojun Zhang , Xichong Liu , Ned S. Wingreen

Protein quality assessment (QA) has played an important role in protein structure prediction. We developed a novel single-model quality assessment method - Qprob. Qprob calculates the absolute error for each protein feature value against…

Quantitative Methods · Quantitative Biology 2016-07-15 Renzhi Cao , Jianlin Cheng

Diverse classes of proteins function through large-scale conformational changes; sophisticated enhanced sampling methods have been proposed to generate these macromolecular transition paths. As such paths are curves in a high-dimensional…

Quantitative Methods · Quantitative Biology 2015-10-27 Sean L. Seyler , Avishek Kumar , Michael F. Thorpe , Oliver Beckstein

Understanding the conformational evolution of $\beta$-amyloid ($A\beta$), particularly the $A\beta_{42}$ isoform, is fundamental to elucidating the pathogenic mechanisms underlying Alzheimer's disease. However, existing end-to-end deep…

Machine Learning · Computer Science 2026-02-24 Qianfeng Yu , Ningkang Peng , Yanhui Gu

Navigating the ultrasound (US) probe to the standardized imaging plane (SIP) for image acquisition is a critical but operator-dependent task in conventional freehand diagnostic US. Robotic US systems (RUSS) offer the potential to enhance…

Robotics · Computer Science 2024-12-11 Xihan Ma , Mingjie Zeng , Jeffrey C. Hill , Beatrice Hoffmann , Ziming Zhang , Haichong K. Zhang

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…