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This work exploits the zeptoliter sensing volume of electrolyte-filled nanopores to determine, simultaneously and in real time, the approximate shape, volume, charge, rotational diffusion coefficient, and dipole moment of individual…

Time-dependent diffusion behavior is probed over sub-millisecond timescales in a single shot using an NMR static gradient, time-incremented echo train acquisition (SG-TIETA) framework. The method extends the Carr-Purcell-Meiboom-Gill (CPMG)…

Quantitative Methods · Quantitative Biology 2021-03-18 Teddy X. Cai , Nathan H. Williamson , Velencia J. Witherspoon , Rea Ravin , Peter J. Basser

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

We combine stroboscopic laser excitation with stochastic photoactivation and super-resolution fluorescence imaging. This makes it possible to record hundreds of diffusion trajectories of small protein molecules in single bacterial cells…

Quantitative Methods · Quantitative Biology 2010-03-11 Brian P. English , Arash Sanamrad , Stoyan Tankov , Vasili Hauryliuk , Johan Elf

Cardiac computed tomography (CT) has emerged as a major imaging modality for the diagnosis and monitoring of cardiovascular diseases. High temporal resolution is essential to ensure diagnostic accuracy. Limited-angle data acquisition can…

Image and Video Processing · Electrical Eng. & Systems 2025-03-12 Shuo Han , Yongshun Xu , Dayang Wang , Bahareh Morovati , Li Zhou , Jonathan S. Maltz , Ge Wang , Hengyong Yu

In Real-Time, Feedback-Driven Single Particle Tracking methods, measurements of the emission intensity from a labeled, nanometer-scale particle are used in a feedback loop to track the motion of the particle as it moves inside its native…

Optics · Physics 2025-12-03 Md Faysal Hossain , Sean B. Andersson

Single Particle Tracking (SPT) can aid in understanding complex spatio-temporal processes. However, quantifying diffusivity and forces from individual live cell trajectories is complicated by inter- & intra-trajectory kinetic heterogeneity,…

Quantitative Methods · Quantitative Biology 2016-05-19 Christopher P. Calderon

In heterogeneous environments, the diffusivity is not constant but changes with time. It is important to detect changes in the diffusivity from single-particle-tracking trajectories in experiments. Here, we devise a novel method for…

Statistical Mechanics · Physics 2017-12-06 Takuma Akimoto , Eiji Yamamoto

Modern developments in microscopy and image processing are revolutionizing areas of physics, chemistry and biology as nanoscale objects can be tracked with unprecedented accuracy. The goal of single particle tracking is to determine the…

Statistical Mechanics · Physics 2015-06-04 Denis Boyer , David S. Dean , Carlos Mejía-Monasterio , Gleb Oshanin

Analysis of signal fluctuations of a locally fixed probe, caused by molecules diffusing under the probe, can be used to determine diffusion coefficients. Theoretical treatments so far have been limited to point-like particles or to…

Statistical Mechanics · Physics 2015-06-18 Susanne Hahne , Philipp Maass

The usually reported pixel resolution of single pixel imaging (SPI) varies between $32 \times 32$ and $256 \times 256$ pixels falling far below imaging standards with classical methods. Low resolution results from the trade-off between the…

Optics · Physics 2022-06-22 Rafał Stojek , Anna Pastuszczak , Piotr Wróbel , Rafał Kotyński

Tomography of single-particle-resolved detectors is of primary importance for characterizing particle correlations with applications in quantum metrology, quantum simulation and quantum computing. However, it is a non-trivial task in…

In this article, we investigate the dynamics of self-organised suspensions formed by rod-like fd virus colloids. Two methods have been employed for analysing fluorescence microscopy movies: single particle tracking (SPT) in direct space and…

Soft Condensed Matter · Physics 2025-01-10 Eric Grelet , Vincent A. Martinez , Jochen Arlt

Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence…

Subcellular Processes · Quantitative Biology 2019-09-25 Mengqi Xu , Lyanne Valdez , Aysuman Sen , Jennifer L. Ross

Single-photon sources are a fundamental resource in quantum optics. The indistinguishability and purity of photons emitted from different sources are crucial (necessary, essential) properties for many quantum applications to ensure…

Quantum Physics · Physics 2020-07-15 Valérian Thiel , Alex O. C. Davis , Ke Sun , Peru D'Ornellas , Xian-Min Jin , Brian J. Smith

We apply a pulsed-light interferometer to measure both the intensity and the phase of light that is transmitted through a strongly scattering disordered material. From a single set of measurements we obtain the time-resolved intensity,…

Disordered Systems and Neural Networks · Physics 2009-11-10 I. M. Vellekoop , P. Lodahl , A. Lagendijk

We follow the trajectories of phase singularities at nulls of intensity in the speckle pattern of waves transmitted through random media as the frequency of the incident radiation is scanned in microwave experiments and numerical…

Disordered Systems and Neural Networks · Physics 2015-06-19 Xiaojun Cheng , Yitzchak Lockerman , Azriel Z. Genack

Single photon sources (SPSs) based on quantum emitters hold promise in quantum radiometry as metrology standard for photon fluxes at the low light level. Ideally this requires control over the photon flux in a wide dynamic range,…

Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion,…

General Physics · Physics 2015-06-04 Lazar L. Kish , Jun Kameoka , Claes G. Granqvist , Laszlo B. Kish

Self-similar solutions of the coherent diffusion equation are derived and measured. The set of real similarity solutions is generalized by the introduction of a nonuniform phase surface, based on the elegant Gaussian modes of optical…

Quantum Physics · Physics 2015-05-19 O. Firstenberg , P. London , D. Yankelev , R. Pugatch , M. Shuker , N. Davidson