Related papers: Photothermal Single Particle Microscopy
The literature on the theory of the photothermal imaging of single nano objects is reviewed. Several models have been devised to describe the signals magnitude, phase and shape in dependence on the experimental and sample parameters…
Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…
We introduce a new, highly sensitive, and simple heterodyne optical method for imaging individual non-fluorescent nano-objects. A two orders of magnitude improvement of the signal is achieved compared to previous methods. This allows for…
Photothermal spectroscopy and microscopy provides a route to measure the spectral and spatial properties of individual nanoscopic absorbers, independent from scattering, extinction, and emission. The approach relies upon use of two light…
We introduce a new, highly sensitive, and simple heterodyne optical method for imaging individual nonfluorescent nanoclusters and nanocrystals. A 2 order of magnitude improvement of the signal is achieved compared to previous methods. This…
The photothermal heterodyne imaging technique enabled studies of individual weakly absorbing nano-objects in various environments. It uses a photo-induced change in the refractive index of the environment. Taking advantage of the dramatic…
Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…
In nanomechanical photothermal absorption spectroscopy and microscopy, the measured substance becomes a part of the detection system itself, inducing a nanomechanical resonance frequency shift upon thermal relaxation. Suspended,…
The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…
The action of a nanoscopic spherically symmetric refractive index profile on a focused Gaussian beam may easily be envisaged as the action of a phase-modifying element, i.e. a lens: Rays traversing the inhomogeneous refractive index field…
Room temperature detection of single quantum emitters has had a broad impact in fields ranging from biophysics to material science, photophysics, or even quantum optics. These experiments have exclusively relied on the efficient detection…
Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…
We present the theoretical and detailed experimental characterizations of Photothermal Heterodyne Imaging. An analytical expression of the photothermal heterodyne signal is derived using the theory of light scattering from a fluctuating…
Understanding light-matter interaction at the nanoscale requires probing the optical properties of matter at the individual nano-absorber level. To this end, we have developed a nanomechanical photothermal sensing platform that can be used…
Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, and nanomaterial investigations. While traditional fluorescence-based methods offer powerful tools for single…
Photothermal microscopy is an emerging tool for measuring light-matter interactions with single-molecule sensitivity. It is generally believed that the spectral acquisition speed in photothermal microscopy is limited by the slow thermal…
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals…
Current optical detection schemes for single molecules require light absorption, either to produce fluorescence or direct absorption signals. This severely limits the range of molecules that can be detected, because most molecules are…
Photothermal heterodyne detection is used to record the first room-temperature absorption spectra of single CdSe/ZnS semiconductor nanocrystals. These spectra are recorded in the high cw excitation regime, and the observed bands are…
Remote sensing of magnetic nanoparticles has exciting applications for magnetic nanoparticle hyperthermia and molecular detection. We introduce, simulate, and experimentally demonstrate an innovation---a sensing coil that is geometrically…