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Yeast cells grown in culture can spontaneously synchronize their respiration, metabolism, gene expression and cell division. Such metabolic oscillations in synchronized cultures reflect single-cell oscillations, but the relationship between…

Genomics · Quantitative Biology 2014-06-16 Nikolai Slavov , David Botstein , Amy Caudy

Single-Cell RNA sequencing (scRNA-seq) measurements have facilitated genome-scale transcriptomic profiling of individual cells, with the hope of deconvolving cellular dynamic changes in corresponding cell sub-populations to better…

Genomics · Quantitative Biology 2021-04-06 Seyednami Niyakan , Ehsan Hajiramezanali , Shahin Boluki , Siamak Zamani Dadaneh , Xiaoning Qian

Surface-consistent deconvolution is a standard processing technique in land data to uniformize the wavelet across all sources and receivers. The required wavelet estimation step is generally done in the homomorphic domain since this is a…

Information Theory · Computer Science 2012-09-18 Roberto H. Herrera , Mirko van der Baan

Until recently, transcriptomics was limited to bulk RNA sequencing, obscuring the underlying expression patterns of individual cells in favor of a global average. Thanks to technological advances, we can now profile gene expression across…

Quantitative Methods · Quantitative Biology 2018-11-30 Miriam Shiffman , William T. Stephenson , Geoffrey Schiebinger , Jonathan Huggins , Trevor Campbell , Aviv Regev , Tamara Broderick

Multimodal single-cell technologies enable the simultaneous collection of diverse data types from individual cells, enhancing our understanding of cellular states. However, the integration of these datatypes and modeling the…

Machine Learning · Computer Science 2023-11-22 Bhavya Mehta , Nirmit Deliwala , Madhav Chandane

Building a virtual cell capable of accurately simulating cellular behaviors in silico has long been a dream in computational biology. We introduce CellFlux, an image-generative model that simulates cellular morphology changes induced by…

The dynamics of gene regulatory networks are often modeled with the assumption of cellular homogeneity. However, this assumption contradicts the plethora of experimental results in a variety of systems, which designates that cell…

Populations and Evolution · Quantitative Biology 2017-08-23 Panagiotis Chrysinas , Michail E. Kavousanakis , Andreas G. Boudouvis

Cell type (e.g. pluripotent cell, fibroblast) is the end result of many complex processes that unfold due to evolutionary, developmental, and transformational stimuli. A cell's phenotype and the discrete, a priori states that define various…

Quantitative Methods · Quantitative Biology 2013-02-05 Bradly Alicea

Cell motility is one of the most fundamental phenomena underlying biological processes that maintain living organisms alive. Here we introduce a simple model to describe the motility of cells which include not only time-correlated internal…

Biological Physics · Physics 2022-07-13 T. N. Azevedo , L. G. Rizzi

Many single-cell observables are highly heterogeneous. A part of this heterogeneity stems from age-related phenomena: the fact that there is a nonuniform distribution of cells with different ages. This has led to a renewed interest in…

Quantitative Methods · Quantitative Biology 2018-04-18 Nash Rochman , Dan Popescu , Sean X. Sun

Metabolic oscillations in single cells underlie the mechanisms behind cell synchronization and cell-cell communication. For example, glycolytic oscillations mediated by biochemical communication between cells may synchronize the pulsatile…

In multicellular systems, the single-cell behaviors should be coordinated consistently with the overall population dynamics and functions. However, the interrelation between single-cell rules and the population-level goal is still elusive.…

Populations and Evolution · Quantitative Biology 2024-09-30 Shuhei A. Horiguchi , Tetsuya J. Kobayashi

This dissertation explores the application of machine learning in molecular biology, focusing on gene expression regulation and cellular behavior at the single-cell level. Using modern neural networks, the research addresses key challenges…

Quantitative Methods · Quantitative Biology 2024-10-01 Yongjian Yang

Celcomen leverages a mathematical causality framework to disentangle intra- and inter- cellular gene regulation programs in spatial transcriptomics and single-cell data through a generative graph neural network. It can learn gene-gene…

The modeling of time series is becoming increasingly critical in a wide variety of applications. Overall, data evolves by following different patterns, which are generally caused by different user behaviors. Given a time series, we define…

Machine Learning · Computer Science 2022-07-13 Wenjie Hu , Jianping Huang , Liang Wu , Yang Yang , Zongtao Liu , Zhanlin Sun , Bingshen Yao , Ke Chen

Bulk gene expression experiments relied on aggregations of thousands of cells to measure the average expression in an organism. Advances in microfluidic and droplet sequencing now permit expression profiling in single cells. This study of…

Methodology · Statistics 2019-07-04 Andrew McDavid , Raphael Gottardo , Noah Simon , Mathias Drton

Multicellular organisms comprise a diverse collection of stable tissues built from different cell types. It remains unclear how large numbers of interacting cells can precisely coordinate their gene expression during tissue…

Biological Physics · Physics 2022-10-31 Matthew Smart , Anton Zilman

Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to…

Analysis of PDEs · Mathematics 2019-02-28 Pierre Gabriel , Shawn P. Garbett , Vito Quaranta , Darren R. Tyson , Glenn F. Webb

Cell-to-cell variability is inherent to numerous biological processes, including cell migration. Quantifying and characterizing the variability of migrating cells is challenging, as it requires monitoring many cells for long time windows…

Biological Physics · Physics 2021-05-06 David B. Brückner , Alexandra Fink , Joachim O. Rädler , Chase P. Broedersz

One of the fundamental problems in biology concerns the method by which a cluster of organisms can regulate the proportion of individuals that perform various roles or modes as if each individual knows a whole situation without a leader. A…

Adaptation and Self-Organizing Systems · Physics 2015-12-21 Mayuko Iwamoto , Daishin Ueyama
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