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The statistical mechanics of heteropolymer structure formation is studied in the context of RNA secondary structures. A designed RNA sequence biased energetically towards a particular native structure (a hairpin) is used to study the…

Statistical Mechanics · Physics 2009-10-31 R. Bundschuh , T. Hwa

DNA methylation (DNAme) is a critical component of the epigenetic regulatory machinery and aberrations in DNAme patterns occur in many diseases, such as cancer. Mapping and understanding DNAme profiles offers considerable promise for…

Synthesis and efficient implementation mRNA strands has been shown to have wide utility, especially recently in the development of COVID vaccines. However, the intrinsic chemical stability of mRNA poses a challenge due to the presence of…

Quantitative Methods · Quantitative Biology 2021-09-28 Ankit Singhal

Consistently predicting biopolymer structure at atomic resolution from sequence alone remains a difficult problem, even for small sub-segments of large proteins. Such loop prediction challenges, which arise frequently in comparative…

Biomolecules · Quantitative Biology 2014-03-05 Rhiju Das

Background and objective: Spatial transcriptomics provides rich spatial context but lacks sufficient resolution for large-scale causal inference. We developed SpeF-Phixer, a spatially extended phi-mixing framework integrating whole-slide…

Quantitative Methods · Quantitative Biology 2025-12-10 Toru Nagasaka , Takaaki Tachibana , Yukari Adachi , Hiroki Kagiyama , Ryota Ito , Mitsugu Fujita , Kimihiro Yamashita , Yoshihiro Kakeji

HiRE-RNA is a simplified, coarse-grained RNA model for the prediction of equilibrium configurations, dynamics and thermodynamics. Using a reduced set of particles and detailed interactions accounting for base-pairing and stacking we show…

Biomolecules · Quantitative Biology 2015-03-10 Tristan Cragnolini , Yoann Laurin , Philippe Derreumaux , Samuela Pasquali

The intricate network of interactions observed in RNA three-dimensional structures is often described in terms of a multitude of geometrical properties, including helical parameters, base pairing/stacking, hydrogen bonding and backbone…

Biomolecules · Quantitative Biology 2015-09-01 Sandro Bottaro , Francesco Di Palma , Giovanni Bussi

RNA secondary structure is an important computational model to understand how genetic variation maps into phenotypic (structural) variation. Evolutionary innovation in RNA structures is facilitated by neutral networks, large connected sets…

Populations and Evolution · Quantitative Biology 2008-01-22 Sumedha , Olivier C Martin , Andreas Wagner

Recent experiments on unzipping of RNA helix-loop structures by force have shown that about 40-base molecules can undergo kinetic transitions between two well-defined `open' and `closed' states, on a timescale = 1 sec [Liphardt et al.,…

Soft Condensed Matter · Physics 2009-11-07 S. Cocco , J. F. Marko , R. Monasson

Recent advancements in deep learning have been primarily driven by the use of large models trained on increasingly vast datasets. While neural scaling laws have emerged to predict network performance given a specific level of computational…

Computer Vision and Pattern Recognition · Computer Science 2023-11-13 Elior Benarous , Sotiris Anagnostidis , Luca Biggio , Thomas Hofmann

Existing state-of-the-art methods that take a single RNA sequence and predict the corresponding RNA secondary-structure are thermodynamic methods. These predict the most stable RNA structure, but do not consider the process of structure…

Biomolecules · Quantitative Biology 2012-07-26 Jeff R. Proctor , Irmtraud M. Meyer

To bridge the gap between the sequences and 3-dimensional (3D) structures of RNAs, some computational models have been proposed for predicting RNA 3D structures. However, the existed models seldom consider the conditions departing from the…

Biological Physics · Physics 2014-09-12 Ya-Zhou Shi , Feng-Hua Wang , Yuan-Yan Wu , Zhi-Jie Tan

Three-dimensional RNA models fitted into crystallographic density maps exhibit pervasive conformational ambiguities, geometric errors and steric clashes. To address these problems, we present enumerative real-space refinement assisted by…

Biomolecules · Quantitative Biology 2012-12-04 Fang-Chieh Chou , Parin Sripakdeevong , Sergey M. Dibrov , Thomas Hermann , Rhiju Das

We present a novel technique of sampling the configurations of helical proteins. Assuming knowledge of native secondary structure, we employ assembly rules gathered from a database of existing structures to enumerate the geometrically…

Soft Condensed Matter · Physics 2009-09-25 Boris Fain , Michael Levitt

AI-based protein structure prediction pipelines, such as AlphaFold2, have achieved near-experimental accuracy. These advanced pipelines mainly rely on Multiple Sequence Alignments (MSAs) as inputs to learn the co-evolution information from…

Biomolecules · Quantitative Biology 2023-10-19 Xiaomin Fang , Fan Wang , Lihang Liu , Jingzhou He , Dayong Lin , Yingfei Xiang , Xiaonan Zhang , Hua Wu , Hui Li , Le Song

Nanotechnology and synthetic biology currently constitute one of the most innovative, interdisciplinary fields of research, poised to radically transform society in the 21st century. This paper concerns the synthetic design of ribonucleic…

Biomolecules · Quantitative Biology 2015-05-29 Ivan Dotu , Juan Antonio Garcia-Martin , Betty L. Slinger , Vinodh Mechery , Michelle M. Meyer , Peter Clote

Background: One-dimensional protein structures such as secondary structures or contact numbers are useful for three-dimensional structure prediction and helpful for intuitive understanding of the sequence-structure relationship. Accurate…

Biomolecules · Quantitative Biology 2007-07-09 Akira R. Kinjo , Ken Nishikawa

Analysis of the sequence-structure relationship in RNA molecules are essential to evolutionary studies but also to concrete applications such as error-correction methodologies in sequencing technologies. The prohibitive sizes of the…

Quantitative Methods · Quantitative Biology 2013-05-31 Vladimir Reinharz , Yann Ponty , Jérôme Waldispühl

Our work is concerned with the generation and targeted design of RNA, a type of genetic macromolecule that can adopt complex structures which influence their cellular activities and functions. The design of large scale and complex…

Biomolecules · Quantitative Biology 2021-02-02 Zichao Yan , William L. Hamilton , Mathieu Blanchette

DNA methylation is a well-studied genetic modification that regulates gene transcription of Eukaryotes. Its alternations have been recognized as a significant component of cancer development. In this study, we use the DNA methylation 450k…

Tissues and Organs · Quantitative Biology 2021-01-05 Shen Jia , Yulin Zhang , Yiming Mao , Jiawei Gao , Yixuan Chen , Yuxuan Jiang , Haochen Luo , Kebo Lv , Jionglong Su