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DNA unzipping by nanopore translocation has implications in diverse contexts, from polymer physics to single-molecule manipulation to DNA-enzyme interactions in biological systems. Here we use molecular dynamics simulations and a…

Soft Condensed Matter · Physics 2025-01-29 Antonio Suma , Cristian Micheletti

Tethered particle motion experiments are versatile single-molecule techniques enabling one to address in vitro the molecular properties of DNA and its interactions with various partners involved in genetic regulations. These techniques…

Biological Physics · Physics 2019-09-05 Manoel Manghi , Nicolas Destainville , Annaël Brunet

Data-driven modeling is useful for reconstructing nonlinear dynamical systems when the underlying process is unknown or too expensive to compute. Having reliable uncertainty assessment of the forecast enables tools to be deployed to predict…

Methodology · Statistics 2023-11-01 Mengyang Gu , Yizi Lin , Victor Chang Lee , Diana Qiu

Recent experiments showed that multiple copies of the molecular machine RNA polymerase (RNAP) can efficiently synthesize mRNA collectively in the active state of the promoter. However, environmentally-induced promoter repression results in…

Subcellular Processes · Quantitative Biology 2021-12-01 Purba Chatterjee , Nigel Goldenfeld , Sangjin Kim

A method is proposed to select the suitable sets of potential parameters for a one-dimensional mesoscopic Hamiltonian model, first introduced to describe the DNA melting transition and later extended to investigate thermodynamic and…

Soft Condensed Matter · Physics 2020-12-09 Marco Zoli

We study the effect of random forces on a double stranded DNA in unzipping the two strands, analogous to the problem of an adsorbed polymer under a random force. The ground state develops bubbles of various lengths as the random force…

Soft Condensed Matter · Physics 2007-05-23 Rajeev Kapri , Somendra M. Bhattacharjee

We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two state manner under the action of mechanical force. We apply path thermodynamics to derive analytical expressions for the average…

Statistical Mechanics · Physics 2009-02-25 Maria Manosas , Alessandro Mossa , Nuria Forns , Josep Maria Huguet , Felix Ritort

We considered a dsDNA polymer in which distribution of bases are random at the base pair level but ordered at a length of 18 base pairs and calculated its force elongation behaviour in the constant extension ensemble. The unzipping force…

Soft Condensed Matter · Physics 2007-05-23 Navin Singh , Yashwant Singh

Eukaryotic transcription generally occurs in bursts of activity lasting minutes to hours; however, state-of-the-art measurements have revealed that many of the molecular processes that underlie bursting, such as transcription factor binding…

Subcellular Processes · Quantitative Biology 2020-12-21 Nicholas C. Lammers , Yang Joon Kim , Jiaxi Zhao , Hernan G. Garcia

The influence of tension on DNA looping has been studied both experimentally and theoretically in the past. However, different theoretical models have yielded different predictions, leaving uncertainty about their validity. We briefly…

Statistical Mechanics · Physics 2025-01-17 Wout Laeremans , Wouter G Ellenbroek

By extending the classical Peyrard-Bishop model, we are able to obtain a fully analytical description for the mechanical resistance of DNA under stretching at variable values of temperature, number of base pairs and intrachains and…

Soft Condensed Matter · Physics 2022-07-20 G. Florio , G. Puglisi

We apply a recently-developed coarse-grained model of DNA, designed to capture the basic physics of nanotechnological DNA systems, to the study of a `burnt-bridges' DNA motor consisting of a single-stranded cargo that steps processively…

Biological Physics · Physics 2014-11-14 Petr Šulc , Thomas E. Ouldridge , Flavio Romano , Jonathan P. K. Doye , Ard A. Louis

The base pair fluctuations and helix untwisting are examined for a circular molecule. A realistic mesoscopic model including twisting degrees of freedom and bending of the molecular axis is proposed. The computational method, based on path…

Biomolecules · Quantitative Biology 2013-05-30 Marco Zoli

A simple zipper model is introduced, representing in a simplified way, e.g., the folded DNA double helix or hairpin structures in RNA. The double stranded hairpin is connected to a heat bath at temperature $T$ and subject to an external…

Statistical Mechanics · Physics 2024-01-18 P. Werner , A. K. Hartmann , S. N. Majumdar

The metazoan genome is replicated in precise cell lineage specific temporal order. However, the mechanism controlling this orchestrated process is poorly understood as no molecular mechanisms have been identified that actively regulate the…

Subcellular Processes · Quantitative Biology 2014-03-12 Yevgeniy Gindin , Manuel S. Valenzuela , Mirit I. Aladjem , Paul S. Meltzer , Sven Bilke

The twisting deformation of mechanically stretched DNA molecules is studied by a coarse grained Hamiltonian model incorporating the fundamental interactions that stabilize the double helix and accounting for the radial and angular base pair…

Soft Condensed Matter · Physics 2017-12-15 Marco Zoli

We propose a dynamical model for the secondary structure of DNA, which is based on the finite stacking enthalpies used in thermodynamics calculations. In this model, the two strands can separate and the bases are allowed to rotate…

Biological Physics · Physics 2016-08-16 Sahin Buyukdagli , Michaël Sanrey , Marc Joyeux

We study by using Monte Carlo simulations the hysteresis in unzipping and rezipping of a double stranded DNA (dsDNA) by pulling its strands in opposite directions in the fixed force ensemble. The force is increased, at a constant rate from…

Soft Condensed Matter · Physics 2012-10-11 Rajeev Kapri

The development of statistical approaches for the joint modelling of the temporal changes of imaging, biochemical, and clinical biomarkers is of paramount importance for improving the understanding of neurodegenerative disorders, and for…

Applications · Statistics 2018-02-16 Marco Lorenzi , Maurizio Filippone , Daniel C. Alexander , Sebastien Ourselin

Experimental studies of the thermal denaturation of DNA yield a strong indication that the transition is first order. This transition has been theoretically studied since the early sixties, mostly within an approach in which the microscopic…

Statistical Mechanics · Physics 2009-11-07 Y. Kafri , D. Mukamel , L. Peliti