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We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule. These experiments are performed in the low-force regime from…

Biological Physics · Physics 2009-11-13 M. S. Rocha , M. C. Ferreira , O. N. Mesquita

We have performed single molecule stretching experiments and dynamic light scattering (DLS) in order to characterize the interaction between the DNA molecule and the fluorescent stain GelRed. The results from single molecule stretching show…

Biological Physics · Physics 2014-08-14 F. A. P. Crisafuli , E. B. Ramos , M. S. Rocha

While analyzing the persistence length of DNA atomistic simulations Wu et al. [J. Chem. Phys. 142, 125103 (2015)] introduced an empirical formula to account for the observed length-dependence. In particular they found that the persistence…

Soft Condensed Matter · Physics 2021-10-26 Midas Segers , Enrico Skoruppa , Jan A. Stevens , Merijn Vangilbergen , Aderik Voorspoels , Enrico Carlon

The distance-resolved effective interaction potential between two parallel DNA molecules is calculated by computer simulations with explicit tetravalent counterions and monovalent salt. Adding counterions first yields an attractive minimum…

Soft Condensed Matter · Physics 2009-11-10 E. Allahyarov , H. Löwen , G. Gompper

Correlation length exponent $\nu$ for long linear DNA molecules was determined by direct measurement of the average end-to-end distance as a function of the contour length $s$ by means of atomic force microscopy (AFM). Linear DNA, up to…

Soft Condensed Matter · Physics 2009-11-11 Francesco Valle , Melanie Favre , Paolo De Los Rios , Angelo Rosa , Giovanni Dietler

Conformational change of a DNA molecule is frequently observed in multiple biological processes and has been modelled using a chain of strongly coupled oscillators with a nonlinear bistable potential. While the mechanism and properties of…

Biological Physics · Physics 2020-08-07 Soumyadip Banerjee , Kushal Shah , Shaunak Sen

Single T4-DNA molecules were confined in rectangular-shaped channels with a depth of 300 nm and a width in the range 150-300 nm casted in a poly(dimethylsiloxane) nanofluidic chip. The extensions of the DNA molecules were measured with…

Biological Physics · Physics 2009-11-13 Ce Zhang , Fang Zhang , Jeroen A. van Kan , Johan R. C. van der Maarel

Single molecule DNA experiments often generate data from force versus extension measurements involving the tethering of a microsphere to one end of a single DNA molecule while the other is attached to a substrate. We show that the…

Biological Physics · Physics 2015-06-26 Jose Coelho Neto , Ronald Dickman , O. N. Mesquita

The effective DNA-DNA interaction force is calculated by computer simulations with explicit tetravalent counterions and monovalent salt. For overcharged DNA molecules, the interaction force shows a double-minimum structure. The positions…

Biomolecules · Quantitative Biology 2009-11-10 E. Allahyarov , G. Gompper , H. Löwen

The flexibility of short DNA chains is investigated via computation of the average correlation function between dimers which defines the persistence length. Path integration techniques have been applied to confine the phase space available…

Soft Condensed Matter · Physics 2018-10-19 Marco Zoli

Finding a theoretical formula for the persistence length of polyelectrolytes for the whole experimental range of salt concentration is a long standing challenge. Using the Tethered Particle Motion technique, the double-stranded DNA…

Soft Condensed Matter · Physics 2023-06-27 Manoel Manghi , Audrey Denis , Nicolas Destainville , Emmanuel Trizac , Catherine Tardin

For short DNA molecules in crowded environments, we evaluate macroscopic parameters such as the average end-to-end distance and the twist conformation by tuning the strength of the site specific confinement driven by the crowders. The…

Soft Condensed Matter · Physics 2019-06-14 Marco Zoli

DNA is structurally and mechanically altered by the binding of intercalator molecules. Intercalation strongly affects the force-extension behavior of DNA, in particular the overstretching transition. We present a statistical model that…

The mechanical properties of molecules are today captured by single molecule manipulation experiments, so that polymer features are tested at a nanometric scale. Yet devising mathematical models to get further insight beyond the commonly…

DNA stretching experiments are usually interpreted using the worm-like chain model; the persistence length A appearing in the model is then interpreted as the elastic stiffness of the double helix. In fact the persistence length obtained by…

Soft Condensed Matter · Physics 2009-10-30 Philip Nelson

From the per unit length free energy for DNA under tension, we have calculated an effective contour length dependent persistence length for short DNA. This effective persistence length results from the enhanced fluctuations in short DNA. It…

Soft Condensed Matter · Physics 2017-06-07 Pui-Man Lam , Yi Zhen

We derive the single molecule equation of state (force-extension relation) for DNA molecules bearing sliding loops and deflection defects. Analytical results are obtained in the large force limit by employing an analogy with instantons in…

Soft Condensed Matter · Physics 2009-11-11 Igor M. Kulic , H. Mohrbach , V. Lobaskin , R. Thaokar , H. Schiessel

While slowly turning the ends of a single molecule of DNA at constant applied force, a discontinuity was recently observed at the supercoiling transition, when a small plectoneme is suddenly formed. This can be understood as an abrupt…

Biomolecules · Quantitative Biology 2011-03-15 Bryan C. Daniels , Scott Forth , Maxim Y. Sheinin , Michelle D. Wang , James P. Sethna

We present a computational method to evaluate the end-to-end and the contour length distribution functions of short DNA molecules described by a mesoscopic Hamiltonian. The method generates a large statistical ensemble of possible…

Soft Condensed Matter · Physics 2018-07-17 Marco Zoli

The dynamics of a loop in DNA molecules at the denaturation transition is studied by scaling arguments and numerical simulations. The autocorrelation function of the state of complementary bases (either closed or open) is calculated. The…

Statistical Mechanics · Physics 2009-11-11 A. Bar , Y. Kafri , D. Mukamel
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