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Interactions between actin, an anionic polyelectrolyte, and lysozyme, a cationic globular protein, have been examined using a combination of synchrotron small-angle x-ray scattering and molecular dynamics simulations. Lysozyme initially…

Measurements of chain conformation in proteins/polyelectrolytes complexes (lysozyme and PSSNa) show that the crossover observed between an open structure -a chain network crosslinked by the proteins, and a globular one - dense globules of ~…

Soft Condensed Matter · Physics 2009-03-31 Jérémie Gummel , Fabrice Cousin , François Boué

We use Small Angle Neutron Scattering (SANS), with an original analysis method, to obtain both the characteristic sizes and the inner composition of lysozyme-pectin complexes depending on the charge density. Lysozyme is a globular protein…

Soft Condensed Matter · Physics 2010-05-11 Isabelle Schmidt , Fabrice Cousin , Christophe Huchon , François Boué , Monique A. V. Axelos

We have studied the structure of salt-free lysozyme at 293 K and pH 7.8 using molecular simulations and experimental SAXS effective potentials between proteins at three volume fractions, 0.012, 0.033, and 0.12. We found that the structure…

Soft Condensed Matter · Physics 2011-06-06 P. Kowalczyk , A. Ciach , P. A. Gauden , A. P. Terzyk

We present an extended structural study of globular complexes made by mixing a positively charge protein (lysozyme) and a negatively charged polyelectrolyte (PSS). We study the influence of all the parameters that may act on the structure…

Soft Condensed Matter · Physics 2010-07-26 Jérémie Gummel , François Boué , Daniel Clemens , Fabrice Cousin

The process of drying is a simple physical mechanism that drives a system to relax from one equilibrium point to another. The native states of the constituent particles in the droplets can be linked to the emergent morphological patterns…

Soft Condensed Matter · Physics 2021-05-14 Anusuya Pal , Amalesh Gope , Germano S. Iannacchione

The structure and stability of strongly charged complex fibers formed by complexation of a single long semi-flexible polyelectrolyte (PE) chain and many oppositely charged spherical macroions are investigated numerically at the ground-state…

Soft Condensed Matter · Physics 2015-03-18 Hoda Boroudjerdi , Ali Naji , Roland R. Netz

The possibilities to tune the structure of solid like material resulting from arrested spinodal decomposition is investigated using a system composed of lysozyme, a globular protein, dispersed in a water solution as model system for…

A quantitative understanding of aggregation mechanisms leading to the formation of inorganic nanoparticles (NPs) and protein composites in aqueous media is of paramount interest for colloid chemistry. In particular, the interactions between…

During directional solidification of the solvent in a colloidal suspension, the colloidal particles segregate from the growing solid, forming high-particle-density regions with structure on a hierarchy of length scales ranging from that of…

Soft Condensed Matter · Physics 2011-03-09 Melissa Spannuth , S. G. J. Mochrie , S. S. L. Peppin , J. S. Wettlaufer

The electrostatic complexation between DOTAP-DOPC unilamellar liposomes and an oppositely charged polyelectrolyte (NaPA) has been investigated in a wide range of the liposome surface charge density. We systematically characterized the…

Soft Condensed Matter · Physics 2015-03-11 Simona Sennato , Laura Carlini , Domenico Truzzolillo , Federico Bordi

We reveal an unreported physical mechanism that enables the pre-concentration, sorting and characterization of charged polystyrene nanobeads and liposomes dispersed in a continuous flow within a straight micron-sized channel. Initially, a…

We study by Small Angle Neutron Scattering (SANS) the structure of Hyaluronan -Lysozyme complexes. Hyaluronan (HA) is a polysaccharide of 9 nm intrinsic persistence length that bears one negative charge per disaccharide monomer (Mmol =…

Biological Physics · Physics 2012-10-04 François Boué , Eric Buhler , Fabrice Cousin , Isabelle Grillo , Isabelle Morfin

Aqueous suspensions of Laponite with discotic particles are well-studied and find a wide range of applications in industry. A new direction of their implementation is polymer composites that can exhibit improved physical properties.We have…

Soft Condensed Matter · Physics 2014-06-12 V. Savenko , L. Bulavin , M. Rawiso , N. Lebovka

Polyelectrolytes such as single and double stranded DNA and many synthetic polymers undergo two structural transitions upon increasing the concentration of multivalent salt or molecules. First, the expanded-stretched chains in low…

Soft Condensed Matter · Physics 2009-10-31 Francisco J. Solis , Monica Olvera de la Cruz

A simple extension of existing models for protein crystallisation is described, in which salt ions and charge neutrality are explicitly incorporated. This provides a straightforward explanation for the shape of protein crystallisation…

Soft Condensed Matter · Physics 2007-05-23 Patrick B. Warren

Small angle X-ray scattering (SAXS) studies in poly[2-methoxy-5-(2'-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV) with varying conjugation, and polyethylene dioxythiophene complexed with polystyrene sulfonate (PEDOT-PSS) in different…

Soft Condensed Matter · Physics 2010-11-11 Paramita Kar Choudhury , Debjani Bagchi , C. S. Suchand Sangeeth , Reghu Menon

We demonstrate in this paper the influence of solvent quality on the structure of the semi-dilute solution of a hydrophobic polyelectrolyte, partially sulfonated Poly-Styrene Sulfonate. Two solvents are used: (i) one mixture of water and an…

Soft Condensed Matter · Physics 2016-11-27 Wafa Essafi , Marie Noelle Spiteri , Claudine Williams , François Boué

Inclusion of protein lysozyme molecules in lipidic monoolein cubic phase induces a transition from a $\rm Pn\bar{3}m$ structure to $\rm Im\bar{3}m$ one. Small-angle X-ray scattering (SAXS) method with high intensity synchrotron radiation…

Soft Condensed Matter · Physics 2009-11-11 S. Tanaka , S. Maki , M. Ataka

Concentrated aqueous solutions of the protein lysozyme undergo a liquid solid transition upon a temperature quench into the unstable spinodal region below a characteristic arrest temperature of Tf=15C. We use video microscopy and…

Soft Condensed Matter · Physics 2015-05-13 Thomas Gibaud , Peter Schurtenberger
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