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Understanding the molecular mechanisms driving the binding between bio-molecules is a crucial challenge in molecular biology. In this respect, characteristics like the preferentially hydrophobic composition of the binding interfaces, the…

the MIAPE Gel Electrophoresis (MIAPE-GE) guidelines specify the minimum information that should be provided when reporting the use of n-dimensional gel electrophoresis in a proteomics experiment. Developed through a joint effort between the…

A new type of phase separation in the polyelectrolyte solutions consisting of several types of charged macromolecules differing in their degree of ionization is predicted via a general thermodynamic consideration. We show that even a small…

Soft Condensed Matter · Physics 2011-11-09 Igor Erukhimovich , Monica Olvera de la Cruz

Microscopic dynamics of complex fluids in the early stage of spinodal decomposition (SD) is strongly intertwined with the kinetics of structural evolution, which makes a quantitative characterization challenging. In this work, we use x-ray…

We present in this paper an original approach to compute the electrophoretic mobility of rigid nucleo-protein complexes like nucleosomes. This model allows to address theoretically the influence of complex position along DNA, as well as…

Biological Physics · Physics 2016-08-14 Martin Castelnovo , Sébastian Grauwin

Proteinoids are solidified gels made from poly(amino acids) based polymers that exhibit oscillatory electrical activity. It has been proposed that proteinoids are capable of performing analog computing as their electrical activity can be…

Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The…

Genomics · Quantitative Biology 2009-04-23 Cécile Lelong , Mireille Chevallet , Sylvie Luche , Thierry Rabilloud

Electrostatic interactions play a fundamental role in the structure and function of proteins. Due to ionizable amino acid residues present on the solvent-exposed surfaces of proteins, the protein charge is not constant but varies with the…

Biological Physics · Physics 2017-10-27 Anze Losdorfer Bozic , Rudolf Podgornik

Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on…

An electrokinetically-driven deterministic lateral displacement (e-DLD) device is proposed for the continuous, two-dimensional fractionation of suspensions in microfluidic platforms. The suspended species are driven through an array of…

Fluid Dynamics · Physics 2016-05-04 Srinivas Hanasoge , Raghavendra Devendra , Javier F. Diez , German Drazer

The concept of chemical bonding is a crucial aspect of chemistry that aids in understanding the complexity and reactivity of molecules and materials. However, the interpretation of chemical bonds can be hindered by the choice of the…

Computational Physics · Physics 2024-01-09 Toni Oestereich , Ralf Tonner-Zech , Julia Westermayr

Electrophoresis is a motion of charged dispersed particles relative to a fluid in a uniform electric field. The effect is widely used to separate macromolecules, to assemble colloidal structures, to transport particles in nano- and…

Soft Condensed Matter · Physics 2011-01-21 Oleg D. Lavrentovich , Israel Lazo , Oleg P. Pishnyak

This article presents a general approach akin to domain-decomposition methods to solve a single linear PDE, but where each subdomain of a partitioned domain is associated to a distinct variational formulation coming from a mutually…

Numerical Analysis · Mathematics 2017-09-26 Federico Fuentes , Brendan Keith , Leszek Demkowicz , Patrick Le Tallec

Over the last decade, proteomic analysis of single cells by mass spectrometry transitioned from an uncertain possibility to a set of robust and rapidly advancing technologies supporting the accurate quantification of thousands of proteins.…

Quantitative Methods · Quantitative Biology 2026-04-14 Nikolai Slavov

We put forward the idea of establishing a novel interdisciplinary field of research at the interface between quantum mechanics and proteomics. The new field, called quantum proteomics, is defined as the large-scale study of the electronic…

Biomolecules · Quantitative Biology 2011-08-01 Fabio Pichierri

Phase separation of intrinsically disordered proteins is important for the formation of membraneless organelles, or biomolecular condensates, which play key roles in the regulation of biochemical processes within cells. In this work, we…

Soft Condensed Matter · Physics 2020-03-18 Antonia Statt , Helena Casademunt , Clifford P. Brangwynne , Athanassios Z. Panagiotopoulos

Lattice models are crucial for studying thermodynamic properties in many physical, biological and chemical systems. We investigate Lattice Restricted Primitive Model (LRPM) of electrolytes with different discretization parameters in order…

Statistical Mechanics · Physics 2009-11-11 Maxim N. Artyomov , Anatoly B. Kolomeisky

In multi-phase fluid flow, fluid-structure interaction, and other applications, partial differential equations (PDEs) often arise with discontinuous coefficients and singular sources (e.g., Dirac delta functions). These complexities arise…

Numerical Analysis · Mathematics 2019-07-24 Chung-Nan Tzou , Samuel Stechmann

We study the electrical double layer at the interface between a protein crystal and a salt solution or a dilute solution of protein, and estimate the double layer's contribution to the interfacial tension of this interface. This…

Soft Condensed Matter · Physics 2009-11-07 R. P. Sear , P. B. Warren

Electrochemical energy systems rely on particulate porous electrodes to store or convert energies. While the three-dimensional porous structures were introduced to maximize the interfacial area for better overall performance of the system,…

Applied Physics · Physics 2021-02-16 Shubham Agrawal , Peng Bai