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Transcription factor binding to the surface of DNA regulatory regions is one of the primary causes of regulating gene expression levels. A probabilistic approach to model protein-DNA interactions at the sequence level is through Position…

Biological Physics · Physics 2015-11-18 Jacob Clifford , Christoph Adami

Precise temporal coordination of gene expression is crucial for many developmental processes. One central question in developmental biology is how such coordinated expression patterns are robustly controlled. During embryonic development of…

Molecular Networks · Quantitative Biology 2015-05-14 Akihiko Nakajima , Takako Isshiki , Kunihiko Kaneko , Shuji Ishihara

For short DNA molecules in crowded environments, we evaluate macroscopic parameters such as the average end-to-end distance and the twist conformation by tuning the strength of the site specific confinement driven by the crowders. The…

Soft Condensed Matter · Physics 2019-06-14 Marco Zoli

Recent studies have consistently inferred high rates of adaptive molecular evolution between Drosophila species. At the same time, the Drosophila genome evolves under different rates of recombination, which results in partial genetic…

Populations and Evolution · Quantitative Biology 2014-09-09 Stephan Schiffels , Michael Lässig , Ville Mustonen

The rate of recombination affects the mode of molecular evolution. In high-recombining sequence, the targets of selection are individual genetic loci; under low recombination, selection collectively acts on large, genetically linked genomic…

Populations and Evolution · Quantitative Biology 2017-11-30 Stephan Schiffels , Ville Mustonen , Michael Lässig

Drosophila Y chromosomes are composed entirely of silent heterochromatin, while male X chromosomes have highly accessible chromatin and are hypertranscribed due to dosage compensation. Here, we dissect the molecular mechanisms and…

Molecular and genetic definitions of human-specific changes to genomic regulatory networks (GRNs) contributing to development of unique to human phenotypes remain a highly significant challenge. Genome-wide proximity placement analysis of…

Genomics · Quantitative Biology 2025-12-30 Gennadi Glinsky

We present a general methodology in order to build mathematical models of genetic regulatory networks. This approach is based on the mass action law and on the Jacob and Monod operon model. The mathematical models are built symbolically by…

Quantitative Methods · Quantitative Biology 2009-09-24 Rui Dilão , Daniele Muraro

Gene expression levels are important molecular quantitative traits that link genotypes to molecular functions and fitness. In Drosophila, population-genetic studies in recent years have revealed substantial adaptive evolution at the genomic…

Populations and Evolution · Quantitative Biology 2015-04-06 Armita Nourmohammad , Joachim Rambeau , Torsten Held , Johannes Berg , Michael Lassig

Binding interactions between proteins and other molecules mediate numerous cellular processes, including metabolism, signaling, and regulation of gene expression. These interactions evolve in response to changes in the protein's chemical or…

Populations and Evolution · Quantitative Biology 2015-02-19 Michael Manhart , Alexandre V. Morozov

Cells regulate fates and complex body plans using spatiotemporal signaling cascades that alter gene expression. Enhancers, short DNA sequences (50-150 base pairs), help coordinate these cascades by attracting regulatory proteins to enhance…

Molecular Networks · Quantitative Biology 2024-02-15 Lucas Hedström , Ralf Metzler , Ludvig Lizana

Comparative genomics methods are widely used to aid the functional annotation of non coding DNA regions. However, aligning non coding sequences requires new algorithms and strategies, in order to take into account extensive rearrangements…

Genomics · Quantitative Biology 2007-10-09 L. Martignetti , M. Caselle , B. Jacq , C. Herrmann

Turnover of regulatory sequence and function is an important part of molecular evolution. But what are the modes of sequence evolution leading to rapid formation and loss of regulatory sites? Here, we show that a large fraction of…

Populations and Evolution · Quantitative Biology 2015-03-19 Armita Nourmohammad , Michael Laessig

Synonymous sites are generally assumed to be subject to weak selective constraint. For this reason, they are often neglected as a possible source of important functional variation. We use site frequency spectra from deep population…

Populations and Evolution · Quantitative Biology 2013-06-14 David S. Lawrie , Philipp W. Messer , Ruth Hershberg , Dmitri A. Petrov

During development of biological organisms, multiple complex structures are formed. In many instances, these structures need to exhibit a high degree of order to be functional, although many of their constituents are intrinsically…

Biological Physics · Physics 2019-03-06 George Courcoubetis , Sammi Ali , Sergey V Nuzhdin , Paul Marjoram , Stephan Haas

Despite significant progress in structural and functional characterization of human genome, understanding of mechanisms underlying the genetic basis of human phenotypic uniqueness remains limited. We report that non-randomly distributed…

Genomics · Quantitative Biology 2025-12-30 Gennadi Glinsky

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik

Gene regulation in higher eukaryotes involves a complex interplay between the gene proximal promoter and distal genomic elements (such as enhancers) which work in concert to drive spatio-temporal expression. The experimental…

Genomics · Quantitative Biology 2008-03-24 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

In early development, regulation of transcription results in precisely positioned and highly reproducible expression patterns that specify cellular identities. How transcription, a fundamentally noisy molecular process, is regulated to…

Subcellular Processes · Quantitative Biology 2018-01-10 Benjamin Zoller , Shawn C. Little , Thomas Gregor

Our understanding of cell division control in bacteria still relies largely on interpreting correlations between phenomenological variables, with limited connection to the underlying molecular mechanisms. Here, we analytically solve a…

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