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The Photonic Force Microscope (PFM) is an opto-mechanical technique based on an optical trap that can be assumed to probe forces in microscopic systems. This technique has been used to measure forces in the range of pico- and femto-Newton,…

Data Analysis, Statistics and Probability · Physics 2011-11-10 Giorgio Volpe , Giovanni Volpe , Dmitri Petrov

We report a new spin manipulation protocol for periodically reversing the sample magnetization for Magnetic Resonance Force Microscopy. The protocol modulates the microwave excitation frequency synchronously with the position of the…

Mesoscale and Nanoscale Physics · Physics 2011-12-12 K. C. Fong , P. Banerjee , Yu. Obukhov , D. V. Pelekhov , P. C. Hammel

Significance: Measuring cerebral blood flow (CBF) is crucial for diagnosing various cerebral diseases. An affordable, wearable, and fiber-free continuous-wave speckle contrast flowmetry (CW-DSCF) technique has been developed for continuous…

Quantitative Methods · Quantitative Biology 2025-02-13 Chaebeom Yeo , Xuhui Liu , Mehrana Mohtasebi , Faezeh Akbari , Faraneh Fathi , Guoqiang Yu

Atomic force microscopy (AFM) with molecule-functionalized tips has emerged as the primary experimental technique for probing the atomic structure of organic molecules on surfaces. Most experiments have been limited to nearly planar…

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

Biological Physics · Physics 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

The control of ultrafast molecular rotational motion has benefited from the development of innovative techniques in strong-field laser physics. Here, we theoretically demonstrate a novel type of coherent control by inducing rotation of an…

Chemical Physics · Physics 2018-08-27 Alec Owens , Andrey Yachmenev , Jochen Küpper

Cell stiffness is a key determinant of how cells deform, migrate, and adapt to mechanically restrictive environments, yet existing single-cell stiffness assays remain difficult to combine with molecular analysis and downstream functional…

Cell Behavior · Quantitative Biology 2026-04-02 Yuhao Zhang , Luyao Zhao , Zhengfu Huang , Kenan Song , Xianqiao Wang , Xianyan Chen , Jin Xie , Yiping Zhao , He Li , Leidong Mao , Yong Teng , Yang Liu

We present imaging and force spectroscopy measurements of DNA molecules adsorbed on functionalized mica. By means of Non-Contact mode AFM (NC-AFM) in Ultra High Vacuum (UHV), the frequency shift (\Delta f) versus separation (z) curves were…

Biological Physics · Physics 2007-12-11 Giovanni Di Santo , Susana Tobenas , Jozef Adamcik , Giovanni Dietler

Current efforts in the biomedical sciences and related interdisciplinary fields are focused on gaining a molecular understanding of health and disease, which is a problem of daunting complexity that spans many orders of magnitude in…

Quantitative Methods · Quantitative Biology 2014-01-24 Julián Candia , Jayanth R. Banavar , Wolfgang Losert

Atomic Force Microscopy (AFM) operating in the frequency modulation mode with a metal tip functionalized with a CO molecule images the internal structure of molecules with an unprecedented resolution. The interpretation of these images is…

Materials Science · Physics 2022-12-29 Jaime Carracedo-Cosme , Rubén Pérez

Molecular-scale computation is crucial for smart materials and nanoscale devices, yet creating single-molecule systems capable of complex computations remains challenging. We present a theoretical framework for a single-molecule computer…

Statistical Mechanics · Physics 2024-10-01 Zhongmin Zhang , Zhiyue Lu

We demonstrate that a bichromatic standing-wave laser field can exert a significantly larger force on a molecule than ordinary radiation pressure. Our experiment measures the deflection of a pulsed supersonic beam of CaF molecules by a…

Atomic Physics · Physics 2018-08-22 S. E. Galica , L. Aldridge , D. J. McCarron , E. E. Eyler , P. L Gould

Biomolecules exhibit dynamic conformations critical to their functions, yet observing these processes at the single-molecule level under native conditions remains a formidable challenge. While surface immobilization has been widely used to…

Instrumentation and Detectors · Physics 2025-12-22 Funing Liu , Qitao Hu , Anton Sabantsev , Giovanni Di Muccio , Shuangshuang Zeng , Mauro Chinappi , Sebastian Deindl , Zhen Zhang

Single-molecule biophysics has transformed our understanding of the fundamental molecular processes involved in living biological systems, but also of the fascinating physics of life. Far more exotic than a collection of exemplars of soft…

Biological Physics · Physics 2017-09-08 Helen Miller , Zhaokun Zhou , Jack Shepherd , Adam J. M. Wollman , Mark C. Leake

Modern parallel computing devices, such as the graphics processing unit (GPU), have gained significant traction in scientific and statistical computing. They are particularly well-suited to data-parallel algorithms such as the particle…

Computation · Statistics 2015-06-12 Lawrence M. Murray , Anthony Lee , Pierre E. Jacob

The microfluidic probe (MFP) is a non-contact, scanning microfluidic technology for local (bio)chemical processing of surfaces based on hydrodynamically confining nanoliter volumes of liquids over tens of micrometers. We present here a…

Tissues and Organs · Quantitative Biology 2014-08-19 J. F. Cors , R. D. Lovchik , E. Delamarche , G. V. Kaigala

Extremely fast rotating molecules carrying significantly more energy in their rotation than in any other degree of freedom are known as "super rotors". It has been speculated that super rotors may exhibit a number of unique properties.…

Optics · Physics 2013-06-11 Aleksey Korobenko , Alexander A. Milner , Valery Milner

Biophysical force spectroscopy tools - for example optical tweezers, magnetic tweezers, atomic force microscopy, - have been used to study elastic, mechanical, conformational and dynamic properties of single biological specimens from single…

Biomolecules · Quantitative Biology 2016-06-09 Zhaokun Zhou , Mark C. Leake

For over three decades, scanning probe microscopy (SPM) has been a key method for exploring material structures and functionalities at nanometer and often atomic scales in ambient, liquid, and vacuum environments. Historically, SPM…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester