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Widefield calcium imaging has recently emerged as a powerful experimental technique to record coordinated large-scale brain activity. These measurements present a unique opportunity to characterize spatiotemporal coherent structures that…

Neurons and Cognition · Quantitative Biology 2021-09-07 Nathaniel J Linden , Dennis R Tabuena , Nicholas A Steinmetz , William J Moody , Steven L Brunton , Bingni W Brunton

Calcium imaging has become a fundamental neural imaging technique, aiming to recover the individual activity of hundreds of neurons in a cortical region. Current methods (mostly matrix factorization) are aimed at detecting neurons in the…

Applications · Statistics 2019-02-11 Gal Mishne , Adam S. Charles

Calcium imaging has emerged as a workhorse method in neuroscience to investigate patterns of neuronal activity. Instrumentation to acquire calcium imaging movies has rapidly progressed and has become standard across labs. Still, algorithms…

Quantitative Methods · Quantitative Biology 2019-06-03 Quico Spaen , Dorit S. Hochbaum , Roberto Asín-Achá

In this paper we present a decomposition algorithm for computation of the spatial-temporal optical flow of a dynamic image sequence. We consider several applications, such as the extraction of temporal motion features and motion detection…

Computer Vision and Pattern Recognition · Computer Science 2017-01-31 Aniello Raffale Patrone , Otmar Scherzer

Non-invasive observation of spatiotemporal neural activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience. We developed a real-time volumetric and multispectral optoacoustic tomography…

Neurons and Cognition · Quantitative Biology 2015-01-13 Gali Sela , Antonella Lauri , X. Luís Deán-Ben , Moritz Kneipp , Vasilis Ntziachristos , Shy Shoham , Gil G. Westmeyer , Daniel Razansky

Optical flow, inspired by the mechanisms of biological visual systems, calculates spatial motion vectors within visual scenes that are necessary for enabling robotics to excel in complex and dynamic working environments. However, current…

Most of the functions performed by astrocytes in brain information processing are related to calcium waves. Experimental studies involving calcium waves present discrepant results, leading to gaps in the full understanding of the functions…

Biological Physics · Physics 2021-07-09 Thais Appelt Peres Bartiê , Leonel Teixeira Pinto

In the past few years, new technologies in the field of neuroscience have made it possible to simultaneously image activity in large populations of neurons at cellular resolution in behaving animals. In mid-2016, a huge repository of this…

Applications · Statistics 2017-03-22 Ashley Petersen , Noah Simon , Daniela Witten

A fundamental challenge in calcium imaging has been to infer the timing of action potentials from the measured noisy calcium fluorescence traces. We systematically evaluate a range of spike inference algorithms on a large benchmark dataset…

Optical imaging of genetically encoded calcium indicators is a powerful tool to record the activity of a large number of neurons simultaneously over a long period of time from freely behaving animals. However, determining the exact time at…

Applications · Statistics 2021-03-08 Tong Shen , Kevin Johnston , Gyorgy Lur , Michele Guindani , Hernando Ombao , Zhaoxia Yu

Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important. Here we apply a…

Neurons and Cognition · Quantitative Biology 2016-12-23 Noah J. Apthorpe , Alexander J. Riordan , Rob E. Aguilar , Jan Homann , Yi Gu , David W. Tank , H. Sebastian Seung

We present a structured matrix factorization approach to analyzing calcium imaging recordings of large neuronal ensembles. Our goal is to simultaneously identify the locations of the neurons, demix spatially overlapping components, and…

Neurons and Cognition · Quantitative Biology 2014-09-11 Eftychios A. Pnevmatikakis , Yuanjun Gao , Daniel Soudry , David Pfau , Clay Lacefield , Kira Poskanzer , Randy Bruno , Rafael Yuste , Liam Paninski

Robust velocity and position estimation is crucial for autonomous robot navigation. The optical flow based methods for autonomous navigation have been receiving increasing attentions in tandem with the development of micro unmanned aerial…

Robotics · Computer Science 2018-12-06 Chen Wang , Tete Ji , Thien-Minh Nguyen , Lihua Xie

Individual Neurons in the nervous systems exploit various dynamics. To capture these dynamics for single neurons, we tune the parameters of an electrophysiological model of nerve cells, to fit experimental data obtained by calcium imaging.…

Quantitative Methods · Quantitative Biology 2017-11-07 Magdalena Fuchs , Manuel Zimmer , Radu Grosu , Ramin M. Hasani

Optical flow is a method aimed at predicting the movement velocity of any pixel in the image and is used in medicine and biology to estimate flow of particles in organs or organelles. However, a precise optical flow measurement requires…

Image and Video Processing · Electrical Eng. & Systems 2021-02-16 Adrian Shajkofci , Michael Liebling

We extend the concept of optical flow with spatiotemporal regularisation to a dynamic non-Euclidean setting. Optical flow is traditionally computed from a sequence of flat images. The purpose of this paper is to introduce variational motion…

Optimization and Control · Mathematics 2014-06-26 Clemens Kirisits , Lukas F. Lang , Otmar Scherzer

Understanding how neurons coordinate their activity is a fundamental question in neuroscience, with implications for learning, memory, and neurological disorders. Calcium imaging has emerged as a powerful method to observe large-scale…

Methodology · Statistics 2026-03-03 Laura D'Angelo , Francesco Denti , Antonio Canale , Michele Guindani

Existing optical flow methods make generic, spatially homogeneous, assumptions about the spatial structure of the flow. In reality, optical flow varies across an image depending on object class. Simply put, different objects move…

Computer Vision and Pattern Recognition · Computer Science 2016-04-12 Laura Sevilla-Lara , Deqing Sun , Varun Jampani , Michael J. Black

In moving camera videos, motion segmentation is commonly performed using the image plane motion of pixels, or optical flow. However, objects that are at different depths from the camera can exhibit different optical flows even if they share…

Computer Vision and Pattern Recognition · Computer Science 2015-11-06 Manjunath Narayana , Allen Hanson , Erik Learned-Miller

Optical flow provides information on relative motion that is an important component in many computer vision pipelines. Neural networks provide high accuracy optical flow, yet their complexity is often prohibitive for application at the edge…

Computer Vision and Pattern Recognition · Computer Science 2023-07-13 Yannick Schnider , Stanislaw Wozniak , Mathias Gehrig , Jules Lecomte , Axel von Arnim , Luca Benini , Davide Scaramuzza , Angeliki Pantazi
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