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Problems of search and recognition appear over different scales in biological systems. In this review we focus on the challenges posed by interactions between proteins, in particular transcription factors, and DNA and possible mechanisms…

Biomolecules · Quantitative Biology 2015-03-19 M. Sheinman , O. Bénichou , Y. Kafri , R. Voituriez

Specific binding of proteins to DNA is one of the most common ways in which gene expression is controlled. Although general rules for the DNA-protein recognition can be derived, the ambiguous and complex nature of this mechanism precludes a…

Biomolecules · Quantitative Biology 2007-12-17 E. Moroni , M. Caselle , F. Fogolari

Allosteric interactions between molecules bound to DNA at distant locations have been known for a long time. The phenomenon has been studied via experiments and numerical simulations, but a comprehensive understanding grounded in a theory…

Soft Condensed Matter · Physics 2018-11-14 Jaspreet Singh , Prashant K. Purohit

Statistical-mechanical lattice models for protein-DNA binding are well established as a method to describe complex ligand binding equilibriums measured in vitro with purified DNA and protein components. Recently, a new field of applications…

Biological Physics · Physics 2014-08-27 Vladimir B. Teif , Karsten Rippe

We review statistical-mechanical theories of single-molecule micromanipulation experiments on nucleic acids. First, models for describing polymer elasticity are introduced. We then review how these models are used to interpret…

Soft Condensed Matter · Physics 2007-05-23 S. Cocco , J. F. Marko , R. Monasson

Brownian motion provides access to hydrodynamic properties of nanoscale objects independent of their optical resolvability. Here, we present a diffusion-based approach to infer effective particle size distributions of DNA-functionalized…

Several recent experiments suggest that sharply bent DNA has a surprisingly high bending flexibility, but the cause of this flexibility is poorly understood. Although excitation of flexible defects can explain these results, whether such…

Biological Physics · Physics 2015-12-22 Peiwen Cong , Liang Dai , Hu Chen , Johan R. C. van der Maarel , Patrick S. Doyle , Jie Yan

Particles with ligand-receptor contacts bind and unbind fluctuating "legs" to surfaces, whose fluctuations cause the particle to diffuse. Quantifying the diffusion of such "nanoscale caterpillars" is a challenge, since binding events often…

Soft Condensed Matter · Physics 2022-03-21 Sophie Marbach , Jeana Aojie Zheng , Miranda Holmes-Cerfon

The growing interest for comparing protein internal dynamics owes much to the realization that protein function can be accompanied or assisted by structural fluctuations and conformational changes. Analogously to the case of functional…

Biomolecules · Quantitative Biology 2012-12-19 C. Micheletti

Fluorescence microscopy reveals that the contents of many (membrane-free) nuclear "bodies" exchange rapidly with the soluble pool whilst the underlying structure persists; such observations await a satisfactory biophysical explanation. To…

Biological Physics · Physics 2017-04-26 C. A. Brackley , B. Liebchen , D. Michieletto , F. Mouvet , P. R. Cook , D. Marenduzzo

Allosteric interactions in DNA are crucial for various biological processes. These interactions are quantified by measuring the change in free energy as a function of the distance between the binding sites for two ligands. Here we show that…

Biological Physics · Physics 2018-05-15 Jaspreet Singh , Prashant K. Purohit

Most of the anticancer drugs bind to double-stranded DNA (dsDNA) by intercalative-binding mode. Although experimental studies have become available recently, a molecular-level understanding of the interactions between the drug and dsDNA…

Soft Condensed Matter · Physics 2021-12-06 Anil Kumar Sahoo , Biman Bagchi , Prabal K. Maiti

DNA bending is biologically important for genome regulation and is relevant to a range of nanotechnological systems. Recent results suggest that sharp bending is much easier than implied by the widely-used worm-like chain model; many of…

Biomolecules · Quantitative Biology 2015-07-01 Ryan M. Harrison , Flavio Romano , Thomas E. Ouldridge , Ard A. Louis , Jonathan P. K. Doye

There is increasing evidence that protein binding to specific sites along DNA can activate the reading out of genetic information without coming into direct physical contact with the gene. There also is evidence that these distant but…

Subcellular Processes · Quantitative Biology 2019-12-19 William Bialek , Thomas Gregor , Gašper Tkačik

We study the effects of the shear force on the rupture mechanism on a double stranded DNA. Motivated by recent experiments, we perform the atomistic simulations with explicit solvent to obtain the distributions of extension in hydrogen and…

Soft Condensed Matter · Physics 2015-06-17 S. Nath , T. Modi , R. K. Mishra , D. Giri , B. P. Mandal , S. Kumar

Composites of flexible and rigid polymers are ubiquitous in biology and industry alike, yet the physical principles determining their mechanical properties are far from understood. Here, we couple force spectroscopy with large-scale…

The dynamics of particles interacting by key-lock binding of attached biomolecules are studied theoretically. Examples of such systems include DNA-functionalized colloids as well as nanoparticles grafted with antibodies to cell membrane…

Soft Condensed Matter · Physics 2007-05-23 Nicholas A. Licata , Alexei V. Tkachenko

The structure of DNA in the nucleosome core particle is studied using an elastic model that incorporates anisotropy in the bending energetics and twist-bend coupling. Using the experimentally determined structure of nucleosomal DNA [T.J.…

Biomolecules · Quantitative Biology 2009-11-13 Farshid Mohammad-Rafiee , Ramin Golestanian

The damping effect to the DNA bubble is investigated within the Peyrard-Bishop model. In the continuum limit, the dynamics of the bubble of DNA is described by the damped nonlinear Schrodinger equation and studied by means of variational…

Biomolecules · Quantitative Biology 2012-06-25 A. Sulaiman , F. P. Zen , H. Alatas , L. T. Handoko

Experiments indicate that unbinding rates of proteins from DNA can depend on the concentration of proteins in nearby solution. Here we present a theory of multi-step replacement of DNA-bound proteins by solution-phase proteins. For four…

Biomolecules · Quantitative Biology 2015-06-19 Simona Cocco , John F. Marko , Remi Monasson