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This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy. Our results show that by including a model of illumination within the first layers of a deep convolutional neural network, it is…

Image and Video Processing · Electrical Eng. & Systems 2020-04-23 Colin L. Cooke , Fanjie Kong , Amey Chaware , Kevin C. Zhou , Kanghyun Kim , Rong Xu , D. Michael Ando , Samuel J. Yang , Pavan Chandra Konda , Roarke Horstmeyer

Cell-cell adhesion plays a vital role in the development and maintenance of multicellular organisms. One of its functions is regulation of cell migration, such as occurs, e.g. during embryogenesis or in cancer. In this work, we develop a…

Tissues and Organs · Quantitative Biology 2024-03-08 Anna Zhigun , Mabel Lizzy Rajendran

One of the major functions of RecA protein in the cell is to bind single-stranded DNA exposed upon damage, thereby triggering the SOS repair response.We present fluorescence anisotropy measurements at the binding onset, showing enhanced DNA…

Biological Physics · Physics 2010-08-05 Tsvi Tlusty , Roy Bar-Ziv , Albert Libchaber

We investigate the continuous quantum measurement of a superconducting qubit undergoing fluorescence. The fluorescence of the qubit is detected via a phase-preserving heterodyne measurement, giving the fluorescence quadrature signals as two…

Quantum Physics · Physics 2016-08-23 Andrew N. Jordan , Areeya Chantasri , Pierre Rouchon , Benjamin Huard

Raman spectroscopy is widely used across scientific domains to characterize the chemical composition of samples in a non-destructive, label-free manner. Many applications entail the unmixing of signals from mixtures of molecular species to…

A model for autonomous feedback control of particle transport through a large number of channels is introduced. Interactions among the particles can lead to a strong suppression of fluctuations in the particle number statistics. Within a…

Statistical Mechanics · Physics 2016-02-25 Tobias Brandes

In the framework of the signal processing approach to single-atom resonance fluorescence with spectral resolution, we diagrammatically derive an analytical formula for arbitrary-order spectral correlation functions of the scattered fields…

Quantum Physics · Physics 2016-09-21 Vyacheslav N. Shatokhin , Sergei Ya. Kilin

Cellular differentiation is governed by gene regulatory networks, the high-dimensional stochastic biochemical systems that determine the transcriptional landscape and mediate cellular responses to signals and perturbations. Although…

Molecular Networks · Quantitative Biology 2026-04-29 Suryanarayana Maddu , Victor Chardès , Michael J. Shelley

Fluctuation in fluorescence emission of immobilized single molecule is typically ascribed to the chromophore's intrinsic structural conformations and the influence of local environmental factors. Despite extensive research over several…

Chemical Physics · Physics 2024-08-07 Aranyak Sarkar , Vinu Namboodiri , Manoj Kumbhakar

Bringing increasingly complex polyatomic molecules within reach of precision measurement experiments offers fascinating and far-reaching prospects ranging from Earth sciences and astrophysics, to metrology and quantum sciences. Here, we…

The dynamics of nanosystems in solution contain a wealth of information with relevance for diverse fields ranging from materials science to biology and biomedical applications. When nanosystems are marked with fluorophores or strong…

Tracking the coupled motion of electrons and nuclei on their intrinsic timescales is essential to understanding and controlling photochemical transformations. While attosecond techniques have provided unprecedented insight into electronic…

Absorption spectroscopy is widely used in sensing and astronomy to understand molecular compositions on microscopic to cosmological scales. However, typical dispersive spectroscopic techniques require multichannel detection, fundamentally…

The nonlinear interaction of x-rays with matter is at the heart of understanding and controlling ultrafast molecular dynamics from an atom-specific viewpoint, providing new scientific and analytical opportunities to explore the structure…

With the advance of fluorescence imaging technologies, recently cell biologists are able to record the movement of protein vesicles within a living cell. Automatic tracking of the movements of these vesicles become key for qualitative…

Quantitative Methods · Quantitative Biology 2015-06-09 Min Xu

Single-particle tracking allows to infer the motion of single molecules in living cells. When we observe a long trajectory (more than 100 points), it is possible that the particle switches mode of motion over time. Then, fitting a single…

Methodology · Statistics 2018-04-16 Vincent Briane , Charles Kervrann , Myriam Vimond

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Protein function is inherently linked to its localization within the cell, and fluorescent microscopy data is an indispensable resource for learning representations of proteins. Despite major developments in molecular representation…

Quantitative Methods · Quantitative Biology 2022-05-25 Anastasia Razdaibiedina , Alexander Brechalov

Brownian motion provides access to hydrodynamic properties of nanoscale objects independent of their optical resolvability. Here, we present a diffusion-based approach to infer effective particle size distributions of DNA-functionalized…

Analyzing the dynamic changes of cellular morphology is important for understanding the various functions and characteristics of live cells, including stem cells and metastatic cancer cells. To this end, we need to track all points on the…

Computer Vision and Pattern Recognition · Computer Science 2023-03-16 Junbong Jang , Kwonmoo Lee , Tae-Kyun Kim
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