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We address the problem of reconstructing phase-shifting objects from their single shot in-line holograms. We show that a phase-shifting object cannot be reliably recovered from its in-line hologram by non-iterative reconstruction routines,…

Optics · Physics 2015-06-01 Tatiana Latychevskaia , Hans-Werner Fink

We present an image recovery approach to improve amplitude and phase reconstruction from single shot digital holograms, using iterative reconstruction with alternating updates. This approach allows the flexibility to apply different priors…

Optics · Physics 2016-10-04 Dennis J. Lee , Charles A. Bouman , Andrew M. Weiner

A method is described for the reconstruction of the amplitude and phase of the object exit wave function by phase-plate transmission electron microscopy. The proposed method can be considered as in-line holography and requires three images,…

Materials Science · Physics 2017-05-24 B. Gamm , M. Dries , K. Schultheiss , H. Blank , A. Rosenauer , R. R. Schröder , D. Gerthsen

Phase retrieval in inline holography is a fundamental yet ill-posed inverse problem due to the nonlinear coupling between amplitude and phase in coherent imaging. We present a novel off-the-shelf solution that leverages a diffusion model…

Optics · Physics 2025-11-12 Jeongsol Kim , Chanseok Lee , Jongin You , Jong Chul Ye , Mooseok Jang

Here we present practical methods for simulation and reconstruction of in-line digital holograms recorded with plane and spherical waves. The algorithms described here are applicable to holographic imaging of an object exhibiting absorption…

Optics · Physics 2023-06-29 Tatiana Latychevskaia , Hans-Werner Fink

Phase recovery from intensity-only measurements forms the heart of coherent imaging techniques and holography. Here we demonstrate that a neural network can learn to perform phase recovery and holographic image reconstruction after…

Computer Vision and Pattern Recognition · Computer Science 2017-12-13 Yair Rivenson , Yibo Zhang , Harun Gunaydin , Da Teng , Aydogan Ozcan

For this work, we introduced the use of binary amplitude for our proposed complex amplitude encoding of a phase-only hologram. By principle, a complex amplitude in a hologram plane can be represented by the amplitude and its phase. However,…

Image and Video Processing · Electrical Eng. & Systems 2020-04-22 Tomoyoshi Shimobaba , Takayuki Takahashi , Yota Yamamoto , Ikuo Hoshi , Atsushi Shiraki , Takashi Kakue , Tomoyoshi Ito

We present a new four-dimensional phase unwrapping approach for time-lapse quantitative phase microscopy, which allows reconstruction of optically thick objects that are optically thin in a certain temporal point and angular view. We thus…

Optics · Physics 2019-09-04 Gili Dardikman , Gyanendra Singh , Natan T. Shaked

Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

Medical Physics · Physics 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

In both light optics and electron optics, the amplitude of a wave scattered by an object is an observable that is usually recorded in the form of an intensity distribution in a real space image or a diffraction image. In contrast, retrieval…

We present a new method for real- and complex-valued image reconstruction from two intensity measurements made in the Fourier plane: the Fourier magnitude of the unknown image, and the intensity of the interference pattern arising from…

Optics · Physics 2012-03-06 Eliyahu Osherovich , Michael Zibulevsky , Irad Yavneh

A method for direct phase difference reconstruction using single-shot dual-wavelength off-axis digital holography is presented. This approach enables direct imaging of samples with high steps without the need to reconstruct phase images at…

Optics · Physics 2025-07-30 M. Amani , M. Dashtdar

X-ray phase contrast imaging significantly improves the visualization of structures with weak or uniform absorption, broadening its applications across a wide range of scientific disciplines. Propagation-based phase contrast is particularly…

Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…

Analysis of PDEs · Mathematics 2026-05-29 Thorsten Hohage , Milad Karimi , Björn Müller

Electron tomography is a technique used in both materials science and structural biology to image features well below optical resolution limit. In this work, we present a new algorithm for reconstructing the three-dimensional(3D)…

Signal Processing · Electrical Eng. & Systems 2018-07-12 David Ren , Michael Chen , Laura Waller , Colin Ophus

Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

We present a system for measuring the amplitude and phase profiles of the pressure field of a harmonic acoustic wave with the goal of reconstructing the volumetric sound field. Unlike optical holograms that cannot be reconstructed exactly…

Soft Condensed Matter · Physics 2018-08-09 Hillary W. Gao , Kimberly I. Mishra , Annemarie Winters , Sidney Wolin , David G. Grier

Digital holographic microscopy based on Gabor in-line holography is a well-known method to reconstruct both the amplitude and phase of small objects. To reconstruct the image of an object from its hologram, obtained under illumination by…

Optics · Physics 2009-11-04 S. G. Podorov , A. I. Bishop , D. M. Paganin , K. M. Pavlov

Phase retrieval and the twin-image problem in digital in-line holographic microscopy can be resolved by iterative reconstruction routines. However, recovering the phase properties of an object in a hologram needs an object plane to be…

Optics · Physics 2018-12-05 Hanqing Zhang , Tim Stangner , Krister Wiklund , Magnus Andersson
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