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The structural dynamics of macromolecules is important for most microbiological processes, from protein folding to the origins of neurodegenerative disorders. Noninvasive measurements of these dynamics are highly challenging. Recently,…

Using a theoretical model for spontaneous partial DNA unwrapping from histones, we study the transient exposure of protein-binding DNA sites within nucleosomes. We focus on the functional dependence of the rates for site exposure and…

Subcellular Processes · Quantitative Biology 2007-08-21 Wolfram Mobius , Richard A. Neher , Ulrich Gerland

The understanding of mechanisms that control epigenetic changes is an important research area in modern functional biology. Epigenetic modifications such as DNA methylation are in general very stable over many cell divisions. DNA…

Genomics · Quantitative Biology 2019-01-21 Charalampos Kyriakopoulos , Pascal Giehr , Alexander Lück , Jörn Walter , Verena Wolf

We consider how active forces modeled as non-thermal random noise affect the average dynamical properties of a Rouse polymer. As the power spectrum of the noise is not known we keep the analytical treatment as generic as possible and then…

Statistical Mechanics · Physics 2017-05-23 Dino Osmanovic , Yitzhak Rabin

The thermodynamic properties of DNA circular molecules are investigated by a new path integral computational method which treats in the real space the fundamental forces stabilizing the molecule. The base pair and stacking contributions to…

Soft Condensed Matter · Physics 2014-11-11 Marco Zoli

We study the activated motion of adsorbed polymers which are driven over a structured substrate by a localized point force.Our theory applies to experiments with single polymers using, for example, tips of scanning force microscopes to drag…

Soft Condensed Matter · Physics 2009-11-11 P. Kraikivski , R. Lipowsky , J. Kierfeld

Recent theoretical predictions on DNA mechanical separation induced by pulling forces are numerically tested within a model in which self-avoidance for DNA strands is fully taken into account. DNA strands are described by interacting pairs…

Statistical Mechanics · Physics 2009-11-07 Enzo Orlandini , Somendra M. Bhattacharjee , Davide Marenduzzo , Amos Maritan , Flavio Seno

We deduced the torque applied on a single stretched and twisted DNA by integrating with respect to force the change in the molecule's extension as it is coiled. While consistent with previous direct measurements of the torque at high forces…

Soft Condensed Matter · Physics 2008-09-04 Francesco Mosconi , Jean-François Allemand , David Bensimon , Vincent Croquette

Magnetic tweezers are used to study the mechanical response under torsion of single nucleosome arrays reconstituted on tandem repeats of 5S positioning sequences. Regular arrays are extremely resilient and can reversibly accommodate a large…

We present a dynamic coarse-graining technique that allows to simulate the mechanical unfolding of biomolecules or molecular complexes on experimentally relevant time scales. It is based on Markov state models (MSM), which we construct from…

Soft Condensed Matter · Physics 2018-08-17 Fabian Knoch , Ken Schäfer , Gregor Diezemann , Thomas Speck

Recent experiments indicated that disorder effect in DNA may lead to a transition of the charge transport mechanism from band resonant tunnelling to thermal activated hopping. In this letter, based on Mott's variable-range hopping theory we…

Soft Condensed Matter · Physics 2013-12-16 Yong-Gang Yang , Peng-Gang Yin , Xin-Qi Li anf YiJing Yan

DNA methylation is an epigenetic mechanism whose important role in development has been widely recognized. This epigenetic modification results in heritable changes in gene expression not encoded by the DNA sequence. The underlying…

Genomics · Quantitative Biology 2017-07-11 Alexander Lück , Pascal Giehr , Jörn Walter , Verena Wolf

A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But,…

Subcellular Processes · Quantitative Biology 2013-10-16 Ajeet K. Sharma , Debashish Chowdhury

A solid-state nanopore can electrophoretically capture a DNA molecule and pull it through in a folded configuration. The resulting ionic current signal indicates where along its length the DNA was captured. A statistical study using an 8 nm…

Biological Physics · Physics 2015-06-11 Mirna Mihovilovic , Nick Hagerty , Derek Stein

Under constant applied force, the separation of double-stranded DNA into two single strands is known to proceed through a series of pauses and jumps. Given experimental traces of constant-force unzipping, we present a method whereby the…

Soft Condensed Matter · Physics 2009-09-29 J. D. Weeks , J. B. Lucks , Y. Kafri , C. Danilowicz , D. R. Nelson , M. Prentiss

We apply Kramers theory to investigate the dissociation of multiple bonds under mechanical force and interpret experimental results for the unfolding/refolding force distributions of an RNA hairpin pulled at different loading rates using…

Soft Condensed Matter · Physics 2009-11-11 M. Manosas , D. Collin , F. Ritort

A versatile approach to modeling the conformations and energetics of DNA loops is presented. The model is based on the classical theory of elasticity, modified to describe the intrinsic twist and curvature of DNA, the DNA bending…

Biological Physics · Physics 2007-05-23 Alexander Balaeff , L. Mahadevan , Klaus Schulten

DNA-spools, structures in which DNA is wrapped and helically coiled onto itself or onto a protein core are ubiquitous in nature. We develop a general theory describing the non-equilibrium behavior of DNA-spools under linear tension. Two…

Soft Condensed Matter · Physics 2009-11-10 I. M. Kulic , H. Schiessel

Controlled movement and manipulation of magnetic micro and nanostructures using magnetic forces can give rise to important applications in biomedecine, diagnostics and immunology. We report controlled magnetophoresis and stretching, in…

Soft Condensed Matter · Physics 2009-11-13 B. Babić , R. Ghai , K. Dimitrov

The twisting deformation of mechanically stretched DNA molecules is studied by a coarse grained Hamiltonian model incorporating the fundamental interactions that stabilize the double helix and accounting for the radial and angular base pair…

Soft Condensed Matter · Physics 2017-12-15 Marco Zoli