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In contrast to engineering applications, in which the structure of control laws are designed to satisfy prescribed function requirements, in biology it is often necessary to infer gene-circuit function from incomplete data on gene-circuit…

Molecular Networks · Quantitative Biology 2007-05-23 Mary J. Dunlop , Michael E. Wall

Gene expression in response to stimuli is regulated by transcription factors (TFs) through feedback loop motifs, aimed at maintaining the desired TF concentration despite uncertainties and perturbations. In this work, we consider a…

Adaptation and Self-Organizing Systems · Physics 2026-02-19 Daniele Proverbio , Giulia Giordano

Turnover of regulatory sequence and function is an important part of molecular evolution. But what are the modes of sequence evolution leading to rapid formation and loss of regulatory sites? Here, we show that a large fraction of…

Populations and Evolution · Quantitative Biology 2015-03-19 Armita Nourmohammad , Michael Laessig

The regulation of a gene depends on the binding of transcription factors to specific sites located in the regulatory region of the gene. The generation of these binding sites and of cooperativity between them are essential building blocks…

Statistical Mechanics · Physics 2007-05-23 Johannes Berg , Stana Willmann , Michael Lässig

Current models for the folding of the human genome see a hierarchy stretching down from chromosome territories, through A/B compartments and TADs (topologically-associating domains), to contact domains stabilized by cohesin and CTCF.…

Biological Physics · Physics 2020-10-02 Peter R. Cook , Davide Marenduzzo

Cellular responses often require the fast activation or repression of specific genes, which depends on Transcription Factors (TFs) that have to quickly find the promoters of these genes within a large genome. Transcription Factors (TFs)…

Subcellular Processes · Quantitative Biology 2015-09-02 Jérôme Cartailler , Jürgen Reingruber

Background: MicroRNAs, post-transcriptional repressors of gene expression, play a pivotal role in gene regulatory networks. They are involved in core cellular processes and their dysregulation is associated to a broad range of human…

Molecular Networks · Quantitative Biology 2012-06-13 Carla Bosia , Matteo Osella , Mariama El Baroudi , Davide Corà , Michele Caselle

Central to the functioning of a living cell is its ability to control the readout or expression of information encoded in the genome. In many cases, a single transcription factor protein activates or represses the expression of many genes.…

Molecular Networks · Quantitative Biology 2013-05-29 Aleksandra M. Walczak , Gapser Tkacik , William Bialek

Specific activator and repressor transcription factors which bind to specific regulator DNA sequences, play an important role in gene activity control. Interactions between genes coding such transcription factors should explain the…

Dynamical Systems · Mathematics 2015-06-26 Gh. I. Mihalas , M. Neamtu , D. Opris , F. Horhat

Transcription Factors (TFs) are proteins crucial for regulating gene expression. Effective regulation requires the TFs to rapidly bind to their correct target, enabling the cell to respond efficiently to stimuli such as nutrient…

Biological Physics · Physics 2025-12-04 Wencheng Ji , Ori Hachmo , Naama Barkai , Ariel Amir

The propagation of noise through parallel regulatory pathways is a characteristic feature of feed-forward loops in genetic networks. Although the contributions of the direct and indirect regulatory pathways of feed-forward loops to output…

Molecular Networks · Quantitative Biology 2025-07-10 Mintu Nandi , Sudip Chattopadhyay , Suman K Banik

We study theoretical ``design principles'' for transcription factor-DNA interaction in bacteria, focusing particularly on the statistical interaction of the transcription factors (TF's) with the genomic background (i.e., the genome without…

Biological Physics · Physics 2009-11-07 Ulrich Gerland , J. David Moroz , Terence Hwa

Recent genome-wide measurements of binding preferences of ~200 transcription regulators in the vicinity of transcription start sites in yeast, have provided a unique insight into the cis- regulatory code of a eukaryotic genome (Venters et…

Biomolecules · Quantitative Biology 2012-04-19 Ariel Afek , David B. Lukatsky

Transcriptional regulatory network inference methods have been studied for years. Most of them relie on complex mathematical and algorithmic concepts, making them hard to adapt, re-implement or integrate with other methods. To address this…

Genomics · Quantitative Biology 2012-08-03 Jianlong Qi , Tom Michoel

MicroRNAs are extensively known for post-transcriptional gene regulation and pattern formation in the embryonic developmental stage. We explore the origin of these spatio-temporal patterns mathematically, considering three different motifs…

Quantitative Methods · Quantitative Biology 2023-07-19 Priya Chakraborty , Sayantari Ghosh

Pre-mRNA splicing relies on the poorly understood dynamic interplay between >150 protein components of the spliceosome. The steps at which splicing can be regulated remain largely unknown. We systematically analyzed the effect of knocking…

Molecular Networks · Quantitative Biology 2015-01-19 Panagiotis Papasaikas , J. Ramon Tejedor , Luisa Vigevani , Juan Valcarcel

To ensure fast gene activation, Transcription Factors (TF) use a mechanism known as facilitated diffusion to find their DNA promoter site. Here we analyze such a process where a TF alternates between 3D and 1D diffusion. In the latter (TF…

Subcellular Processes · Quantitative Biology 2015-05-27 Juergen Reingruber , David Holcman

In the simplest view of transcriptional regulation, the expression of a gene is turned on or off by changes in the concentration of a transcription factor (TF). We use recent data on noise levels in gene expression to show that it should be…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Curtis G Callan , William Bialek

Transcription factors (TFs) regulate gene expression by binding to specific genomic loci determined by DNA sequence. Their sequence specificity is commonly summarized by a consensus binding motif. However, eukaryotic genomes contain…

While coding regions in the genome have a direct interpretation in terms of protein products, significant fractions are non-coding and yet control essential biological functions. Unlike the genetic code, there is no "lookup table" that…