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Many simple RNA viruses enclose their genetic material by a protein shell called the capsid. While the capsid structures are well characterized for most viruses, the structure of RNA inside the shells and the factors contributing to it…

Biological Physics · Physics 2017-11-07 Gonca Erdemci-Tandogan , Henri Orland , Roya Zandi

Simple RNA viruses efficiently encapsulate their genome into a nano-sized protein shell: the capsid. Spontaneous co-assembly of the genome and the capsid proteins is driven predominantly by electrostatic interactions between the negatively…

Biological Physics · Physics 2016-01-06 Gonca Erdemci-Tandogan , Jef Wagner , Paul van der Schoot , Rudolf Podgornik , Roya Zandi

Simple RNA viruses self-assemble spontaneously and encapsulate their genome into a shell called the capsid. This process is mainly driven by the attractive electrostatic interaction between the positive charges on capsid proteins and the…

Biological Physics · Physics 2022-02-22 Sanaz Panahandeh , Siyu Li , Bogdan Dragnea , Roya Zandi

Under many in vitro conditions, some small viruses spontaneously encapsidate a single stranded (ss) RNA into a protein shell called the capsid. While viral RNAs are found to be compact and highly branched because of long distance…

Biological Physics · Physics 2018-01-17 Siyu Li , Gonca Erdemci-Tandogan , Paul van der Schoot , Roya Zandi

During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively…

Biomolecules · Quantitative Biology 2015-02-19 J. D. Perlmutter , M. F. Hagan

Recent studies reveal that certain viruses package a portion of their genome in a manner that mirrors the icosahedral symmetry of the protein container, or capsid. Graph theoretical constraints forbid exact realization of icosahedral…

Soft Condensed Matter · Physics 2007-05-23 Joseph Rudnick , Robijn Bruinsma

Single-stranded RNA viruses efficiently encapsulate their genome into a protein shell called the capsid. Electrostatic interactions between the positive charges in the capsid protein's N-terminal tail and the negatively charged genome have…

Biological Physics · Physics 2021-02-03 Yinan Dong , Siyu Li , Roya Zandi

The formation of a viral capsid -- the highly-ordered protein shell that surrounds the genome of a virus -- is the canonical example of self-assembly. The capsids of many positive-sense RNA viruses spontaneously assemble from in vitro…

Soft Condensed Matter · Physics 2022-06-08 Rees F. Garmann , Aaron M. Goldfain , Vinothan N. Manoharan

The aim of the study is to analyze viruses using parameters obtained from distributions of nucleotide sequences in the viral RNA. Seeking for the input data homogeneity, we analyze single-stranded RNA viruses only. Two approaches are used…

Other Quantitative Biology · Quantitative Biology 2022-04-25 Mykola Husev , Andrij Rovenchak

The packaging of genetic material within a protein shell, called the capsid, marks a pivotal step in the life cycle of numerous single-stranded RNA viruses. Understanding how hundreds, or even thousands, of proteins assemble around the…

Biological Physics · Physics 2024-09-04 Siyu Li , Guillaume Tresset , Roya Zandi

The structural organisation of the viral genome within its protein container, called the viral capsid, is an important aspect of virus architecture. Many single-stranded (ss) RNA viruses organise a significant part of their genome in a…

Biomolecules · Quantitative Biology 2007-05-23 N. Jonoska , R. Twarock

Single-stranded (ss) RNA viruses self-assemble spontaneously in solutions that contain the viral RNA genome molecules and the viral capsid proteins. The self-assembly of empty capsids can be understood on the basis of free energy…

Biological Physics · Physics 2021-08-21 Inbal Mizrahi , Robijn Bruinsma , Joseph Rudnick

Myriad viruses use positive-strand RNA molecules as their genomes. Far from being only a repository of genetic material, viral RNA performs numerous other functions mediated by its physical structure and chemical properties. In this…

Biological Physics · Physics 2024-09-27 Domen Vaupotič , Angelo Rosa , Rudolf Podgornik , Luca Tubiana , Anže Božič

We present a simple kinetic model for the assembly of small single-stranded RNA viruses that can be used to carry out analytical packaging contests between different types of RNA molecules. The RNA selection mechanism is purely kinetic and…

Biological Physics · Physics 2021-02-09 Inbal Mizrahi , Robijn Bruinsma , Joseph Rudnick

Viruses are nanoscale entities containing a nucleic acid genome encased in a protein shell called a capsid, and in some cases surrounded by a lipid bilayer membrane. This review summarizes the physics that govern the processes by which…

Biomolecules · Quantitative Biology 2015-05-20 Jason D Perlmutter , Michael F Hagan

Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins…

Biomolecules · Quantitative Biology 2014-05-15 Jason D. Perlmutter , Cong Qiao , Michael F. Hagan

Although the free energy of a genome packing into a virus is dominated by DNA-DNA interactions, ordering of the DNA inside the capsid is elasticity-driven, suggesting general solutions with DNA organized into spool-like domains. Using…

Soft Condensed Matter · Physics 2024-03-20 Tine Curk , James Daniel Farrell , Jure Dobnikar , Rudolf Podgornik

Many single-stranded (ss) RNA viruses self assemble from capsid protein subunits and the nucleic acid to form an infectious virion. It is believed that the electrostatic interactions between the negatively charged RNA and the positively…

Biological Physics · Physics 2016-08-10 Gonca Erdemci-Tandogan , Jef Wagner , Paul van der Schoot , Rudolf Podgornik , Roya Zandi

We characterize the equilibrium thermodynamics of a thick polymer confined in a spherical region of space. This is used to gain insight into the DNA packaging process. The experimental reference system for the present study is the recent…

Statistical Mechanics · Physics 2007-05-23 Davide Marenduzzo , Cristian Micheletti

Viruses are biological nanosystems with a capsid of protein-made capsomer units that encloses and protects the genetic material responsible for their replication. Here we show how the geometrical constraints of the capsomer-capsomer…

Biological Physics · Physics 2014-12-17 J. M. Gomez Llorente , J. Hernandez-Rojas , J. Breton
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