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In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…

Soft Condensed Matter · Physics 2020-02-25 Lauren E. Altman , David G. Grier

Holographic particle characterization uses quantitative analysis of holographic microscopy data to precisely and rapidly measure the diameter and refractive index of individual colloidal spheres in their native media. When this technique is…

Soft Condensed Matter · Physics 2022-10-19 Rafe Abdulali , Lauren E. Altman , David G. Grier

Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…

We demonstrate precise measurements of the size and refractive index of individual dimpled colloidal spheres using holographic characterization techniques developed for ideal spheres.

Soft Condensed Matter · Physics 2015-10-28 Mark Hannel , Christine Middleton , David G. Grier

Quantitative tracking of features from video images is a basic technique employed in many areas of science. Here, we present a method for the tracking of features that partially overlap, in order to be able to track so-called colloidal…

Soft Condensed Matter · Physics 2016-11-24 Casper van der Wel , Daniela J. Kraft

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…

Optics · Physics 2015-06-03 Frédéric Verpillat , Fadwa Joud , Pierre Desbiolles , Michel Gross

An in-line hologram of a colloidal sphere can be analyzed with the Lorenz-Mie theory of light scattering to measure the sphere's three-dimensional position with nanometer-scale precision while also measuring its diameter and refractive…

Soft Condensed Matter · Physics 2020-12-29 Lauren E. Altman , Rushna Quddus , Fook Chiong Cheong , David G. Grier

The trajectories of colloidal particles driven through a periodic potential energy landscape can become kinetically locked in to directions dictated by the landscape's symmetries. When the landscape is realized with forces exerted by a…

Soft Condensed Matter · Physics 2015-05-14 Ke Xiao , David G. Grier

Particle tracking is a key to single-particle-level confocal microscopy observation of colloidal suspensions, emulsions, and granular matter. The conventional tracking method has not been able to provide accurate information on the size of…

Soft Condensed Matter · Physics 2013-01-31 Mathieu Leocmach , Hajime Tanaka

In-line holographic video microscopy records a wealth of information about the microscopic structure and dynamics of colloidal materials. Powerful analytical techniques are available to retrieve that information when the colloidal particles…

Soft Condensed Matter · Physics 2024-03-19 Jatin Abacousnac , Jasna Brujic , David G. Grier

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo

Holograms of colloidal particles can be analyzed with the Lorenz-Mie theory of light scattering to measure individual particles' three-dimensional positions with nanometer precision while simultaneously estimating their sizes and refractive…

Soft Condensed Matter · Physics 2018-07-04 Mark D. Hannel , Aidan Abdulali , Michael O'Brien , David G. Grier

In this paper, we propose a new algorithm based on radial symmetry center method to track colloidal particles close to contact, where the optical images of the particles start to overlap in digital video microscopy. This overlapping effect…

Instrumentation and Detectors · Physics 2017-10-26 Harun Yücel , Nazmi Turan Okumuşoğlu

How far a particle moves along the optical axis in a holographic optical trap is not simply dictated by the programmed motion of the trap, but rather depends on an interplay of the trap's changing shape and the particle's material…

Soft Condensed Matter · Physics 2019-10-02 Michael J. O'Brien , David G. Grier

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

Holographic microscopy combined with forward modeling and inference allows colloidal particles to be characterized and tracked in three dimensions with high precision. However, current models ignore the effects of optical aberrations on…

Optics · Physics 2021-05-27 Caroline Martin , Brian Leahy , Vinothan Manoharan

The precise tracking of micron sized colloidal particles - held in the vicinity of each other using optical tweezers - is an elegant way to gain information about the particle-particle pair interaction potential. The accuracy of the method,…

Optics · Physics 2017-09-26 Chi Zhang , Georges Brügger , Frank Scheffold

We describe a novel method of tracking the rotational motion of clusters of colloidal particles. Our method utilizes rigid body transfor- mations to determine the rotations of a cluster and extends conventional proven particle tracking…

Soft Condensed Matter · Physics 2011-09-01 Gary L. Hunter , Kazem V. Edmond , Mark T. Elsesser , Eric R. Weeks

Holographic microscopy has developed into a powerful tool for 3D particle tracking, yielding nanometer-scale precision at high frame rates. However, current particle tracking algorithms ignore the effect of the microscope objective on the…

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