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When pulled along its axis, double-stranded DNA elongates abruptly at a force of about 65 pN. Two physical pictures have been developed to describe this overstretched state. The first proposes that strong forces induce a phase transition to…

Statistical Mechanics · Physics 2015-06-25 Stephen Whitelam , Sander Pronk , Phillip L. Geissler

We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we get an…

Biomolecules · Quantitative Biology 2013-05-29 Sebastien Neukirch

In mechanical manipulation experiments, a single DNA molecule overwound at constant force undergoes a discontinuous drop in extension as it buckles and forms a superhelical loop (a plectoneme). Further overwinding the DNA, we observe an…

Biological Physics · Physics 2018-04-23 Andrew Dittmore , Keir C. Neuman

A double stranded DNA molecule under the stress of a pulling force acting on the strand terminals exhibits a partially denatured structure or can be completely unzipped depending the magnitude of the pulling force. A scaling argument for…

Statistical Mechanics · Physics 2009-09-25 Jeff Z. Y. Chen

DNA supercoiling, the under or overwinding of DNA, is a key physical mechanism both participating to compaction of bacterial genomes and making genomic sequences adopt various structural forms. DNA supercoiling may lead to the formation of…

Biological Physics · Physics 2019-06-26 Thibaut Lepage , Ivan Junier

In a recent experiment, a single DNA double helix is stretched and relaxed in the presence of spermidine, a short positive polyelectrolyte, and the pulling force is measured as a function of DNA extension. In a certain range of spermidine…

Soft Condensed Matter · Physics 2009-11-10 R. Zhang , B. I. Shklovskii

A simple model of DNA based on two interacting polymers has been used to study the unzipping of a double stranded DNA subjected to a periodic force. We propose a dynamical transition, where without changing the physiological condition, it…

Biological Physics · Physics 2015-06-11 Sanjay Kumar , Garima Mishra

In recent years, significant progress in understanding the properties of supercoiled DNA has been obtained due to nanotechniques that made stretching and twisting of single molecules possible. Quantitative interpretation of such experiments…

Biomolecules · Quantitative Biology 2015-06-05 Alexey K. Mazur

We present a critique and extension of the mean-field approach to the mechanical pulling transition in bound polymer systems. Our model is motivated by the theoretically and experimentally important examples of adsorbed polymers and…

Soft Condensed Matter · Physics 2009-11-07 Eran A. Mukamel , Eugene I. Shakhnovich

Charge migration along DNA molecules has attracted scientific interest for over half a century. Reports on possible high rates of charge transfer between donor and acceptor through the DNA, obtained in the last decade from solution…

Materials Science · Physics 2009-11-10 Danny Porath , Gianaurelio Cuniberti , Rosa Di Felice

Motivated by the experiments on DNA under torsion, we consider the problem of pulling a polymer out of a potential well by a force applied to one of its ends. If the force is less than a critical value, then the process is activated and has…

Soft Condensed Matter · Physics 2009-10-31 K. L. Sebastian

Inspired by its central role in many biological processes, the transport of biopolymers across nanoscale pores is at the heart of a single-molecule sensing technology aimed at nucleic acid and protein sequencing, as well as biomarker…

Biological Physics · Physics 2025-10-16 Martin Charron , Breeana Elliott , Nada Kerrouri , Liqun He , Vincent Tabard-Cossa

We study the melting of a double stranded DNA in the presence of stretching forces, via 3D Monte-Carlo simulations, exactly solvable models and heuristic arguments. The resulting force-temperature phase diagram is dramatically different for…

Soft Condensed Matter · Physics 2008-07-22 D. Marenduzzo , A. Maritan , E. Orlandini , F. Seno , A. Trovato

We present a mean-field dynamical theory for single semiflexible polymers which can precisely capture, without fitting parameters, recent fluorescence correlation spectroscopy results on single monomer kinetics of DNA strands in solution.…

Soft Condensed Matter · Physics 2009-10-19 Michael Hinczewski , Roland R. Netz

The self-assembly of DNA-coated colloids into highly-ordered structures offers great promise for advanced optical materials. However, control of disorder, defects, melting, and crystal growth is hindered by the lack of a microscopic…

Soft Condensed Matter · Physics 2022-05-18 Fan Cui , Sophie Marbach , Jeana Aojie Zheng , Miranda Holmes-Cerfon , David J. Pine

The twisting deformation of mechanically stretched DNA molecules is studied by a coarse grained Hamiltonian model incorporating the fundamental interactions that stabilize the double helix and accounting for the radial and angular base pair…

Soft Condensed Matter · Physics 2017-12-15 Marco Zoli

The folding transition of biopolymers from the coil to compact structures has attracted wide research interest in the past and is well studied in polymer physics. Recent seminal works on DNA in confined devices have shown that these long…

Soft Condensed Matter · Physics 2023-08-09 Keerthi Radhakrishnan , Sunil P. Singh

We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule. These experiments are performed in the low-force regime from…

Biological Physics · Physics 2009-11-13 M. S. Rocha , M. C. Ferreira , O. N. Mesquita

The requirement to boost the resolution of nanopore-based biosequencing devices necessitates the integration of novel biosensing techniques with reduced sensitivity to background noise. In this article, we probe the signatures of…

Soft Condensed Matter · Physics 2023-05-01 Sahin Buyukdagli

We present a self-contained theory for the mechanical response of DNA in single molecule experiments. Our model is based on a 1D continuum description of the DNA molecule and accounts both for its elasticity and for DNA-DNA electrostatic…

Biomolecules · Quantitative Biology 2015-05-13 N. Clauvelin , B. Audoly , S. Neukirch