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Length-regulation of microtubules (MTs) is essential for many cellular processes. Molecular motors like kinesin 8, which move along MTs and also act as depolymerases, are known as key players in MT dynamics. However, the regulatory…

Subcellular Processes · Quantitative Biology 2012-10-18 Anna Melbinger , Louis Reese , Erwin Frey

In this work we study a microtubule (MT) model, whose length is regulated by the action of processive kinesin motors. We treat the case of infinite processivity, i.e. particle exchange in the bulk is neglected. The exact results can be…

Statistical Mechanics · Physics 2015-06-25 Chikashi Arita , Alexander Lück , Ludger Santen

Many cellular processes rely on the cell's ability to transport material to and from the nucleus. Networks consisting of many microtubules and actin filaments are key to this transport. Recently, the inhibition of intracellular transport…

Subcellular Processes · Quantitative Biology 2020-05-06 Shawn D. Ryan , Zachary McCarthy , Mykhailo Potomkin

Regulating physical size is an essential problem that biological organisms must solve from the subcellular to the organismal scales, but it is not well understood what physical principles and mechanisms organisms use to sense and regulate…

Biological Physics · Physics 2014-07-21 Hui-Shun Kuan , M. D. Betterton

Proteins from the kinesin-8 family promote microtubule (MT) depolymerization, a process thought to be important for the control of microtubule length in living cells. In addition to this MT shortening activity, kinesin 8s are motors that…

Biological Physics · Physics 2015-05-13 L. E. Hough , Anne Schwabe , Matthew A. Glaser , J. Richard McIntosh , M. D. Betterton

Microtubules are highly regulated dynamic elements of the cytoskeleton of eukaryotic cells. One of the regulation mechanisms observed in living cells is the severing by the proteins katanin and spastin. We introduce a model for the dynamics…

Subcellular Processes · Quantitative Biology 2010-03-12 Simon H. Tindemans , Bela M. Mulder

Assembly and disassembly dynamics of microtubules (MTs) is tightly controlled by MT associated proteins. Here, we investigate how plus-end-directed depolymerases of the kinesin-8 family regulate MT depolymerization dynamics. Employing an…

Biological Physics · Physics 2011-11-15 Louis Reese , Anna Melbinger , Erwin Frey

Certain regulatory proteins influence the polymerization dynamics of microtubules by inducing catastrophe with a rate that depends on the microtubule length. Using a discrete formulation, here we show that, for a catastrophe rate…

Biological Physics · Physics 2012-08-27 Vandana Yadav , Sutapa Mukherji

The availability of protein is an important factor for the determination of the size of the mitotic spindle. Involved in spindle-size regulation is kinesin-8, a molecular motor and microtubule (MT) depolymerase, which is known to tightly…

Biological Physics · Physics 2018-04-10 Matthias Rank , Aniruddha Mitra , Louis Reese , Stefan Diez , Erwin Frey

The microtubule cytoskeleton is responsible for sustained, long-range intracellular transport of mRNAs, proteins, and organelles in neurons. Neuronal microtubules must be stable enough to ensure reliable transport, but they also undergo…

Dynamical Systems · Mathematics 2024-03-05 Anna C Nelson , Melissa M Rolls , Maria-Veronica Ciocanel , Scott A McKinley

Biopolymers serve as one-dimensional tracks on which motor proteins move to perform their biological roles. Motor protein phenomena have inspired theoretical models of one-dimensional transport, crowding, and jamming. Experiments studying…

Biological Physics · Physics 2016-05-25 Hui-Shun Kuan , M. D. Betterton

We analyze experimental observations of microtubules undergoing small fluctuations about a "balance point" when mixed in solution of two different kinesin motor proteins, KLP61F and Ncd. It has been proposed that the microtubule movement is…

Cell Behavior · Quantitative Biology 2013-07-24 J. M. Deutsch , Ian P. Lewis

In cells and in vitro assays the number of motor proteins involved in biological transport processes is far from being unlimited. The cytoskeletal binding sites are in contact with the same finite reservoir of motors (either the cytosol or…

Subcellular Processes · Quantitative Biology 2014-09-26 Luca Ciandrini , I. Neri , Jean-Charles Walter , O. Dauloudet , A. Parmeggiani

A theoretical model of stabilization of a microtubule assembly due to microtubule-associated-proteins(MAP) is presented. MAPs are assumed to bind to the microtubule filaments, thus preventing their disintegration following hydrolysis and…

Subcellular Processes · Quantitative Biology 2012-08-27 Bindu S. Govindan , William B. Spillman,

The cytoskeleton is regulated by a plethora of enzymes that influence the stability and dynamics of cytoskeletal filaments. Molecular motors of the kinesin-8 protein family depolymerise microtubules in a length-dependent manner, and…

Subcellular Processes · Quantitative Biology 2014-10-29 Louis Reese , Anna Melbinger , Erwin Frey

Motor proteins that specifically interact with the ends of cytoskeletal filaments can induce filament depolymerization. A phenomenological description of this process is presented. We show that under certain conditions motors dynamically…

Subcellular Processes · Quantitative Biology 2009-11-11 Gernot A. Klein , Karsten Kruse , Gianaurelio Cuniberti , Frank Juelicher

Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this…

Biological Physics · Physics 2024-08-22 Soichi Hirokawa , Heun Jin Lee , Rachel A Banks , Ana I Duarte , Bibi Najma , Matt Thomson , Rob Phillips

The control of biopolymer length is mediated by proteins that localize to polymer ends and regulate polymerization dynamics. Several mechanisms have been proposed to achieve end localization. Here, we propose a novel mechanism by which a…

Biological Physics · Physics 2026-03-12 Amer Al-Hiyasat , Yazgan Tuna , Yin-Wei Kuo , Jonathon Howard

Microtubules are protein polymers that form "molecular highways" for long-range transport within living cells. Molecular motors actively step along microtubules to shuttle cellular materials between the nucleus and the cell periphery; this…

Biomolecules · Quantitative Biology 2016-10-06 Winnie H. Liang , Qiaochu Li , K. M. Rifat Faysal , Stephen J. King , Ajay Gopinathan , Jing Xu

In living cells, motor proteins, such as kinesin and dynein can move processively along microtubule (MT), and also detach from or attach to MT stochastically. Experiments have found that, the traffic of motor might be jammed, and various…

Biological Physics · Physics 2015-03-06 Yunxin Zhang
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