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Fluorescence-lifetime imaging microscopy (FLIM) was applied to investigate the donor distribution in SrTiO3 single crystals. On the surfaces of Nb- and La-doped SrTiO3, structures with different fluorescence intensities and lifetimes were…

Materials Science · Physics 2013-10-23 C. Rodenbücher , T. Gensch , W. Speier , U. Breuer , M. Pilch , H. Hardtdegen , M. Mikulics , E. Zych , R. Waser , K. Szot

Nanoparticle (NP) arrays of noble metals strongly absorb light in the visible to infrared wavelengths through resonant interactions between the incident electromagnetic field and the metal's free electron plasma. Such plasmonic interfaces…

Mesoscale and Nanoscale Physics · Physics 2013-09-06 Miriam Israelowitz , Jennifer Amey , Tao Cong , Radhakrishna Sureshkumar

Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Takashi Hato , Pierre C. Dagher , Evan L. Nichols , Cody J. Smith , Kenneth W. Dunn , Scott Howard

The inherently weak nonlinear optical response of bulk materials remains a fundamental limitation in advancing photonic technologies. Nanophotonics addresses this challenge by tailoring the size and morphology of nanostructures to…

Hyperspectral imaging is gaining attention in the field of disease diagnosis due to its ability to enhance tissue contrast, surpassing the capabilities of conventional brightfield imaging techniques. Typically, histological sections lack…

We numerically simulate, using finite-difference time-domain, the optical properties of silver nano discs deposited on the front surface of silicon solar cells. We explore the effect of each of the parameters of such a system, in order to…

Mesoscale and Nanoscale Physics · Physics 2015-03-19 Bruno Figeys , Ounsi El Daif

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near-fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the…

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…

Instrumentation and Detectors · Physics 2017-08-14 Egidijus Auksorius

Extreme field localization and giant field enhancement are often achieved by using plasmonic nanostructures and metamaterials such as strongly coupled silver nanoparticles.Dielectric particles and structures can focus light beyond the…

Optical field localization at plasmonic tip-sample nanojunctions has enabled high spatial resolution chemical analysis through tip-enhanced linear optical spectroscopies, including Raman scattering and photoluminescence. Here, we illustrate…

Optics · Physics 2021-10-01 Chih-Feng Wang , Patrick Z. El-Khoury

The active tuning of the structural colour in photonic crystals by an electric field represents an effective external stimulus with impact on light transmission manipulation. In this work we present this effect in a photonic crystal device…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

The development of voltage-sensitive fluorescent probes suggests fluorescence lifetime as a promising readout for electrical activity in biological systems. Existing approaches fail to achieve the speed and sensitivity required for voltage…

Optics · Physics 2023-06-27 Adam J. Bowman , Cheng Huang , Mark J. Schnitzer , Mark A. Kasevich

Spatial filtering is an important mechanism to improve the spatial quality of laser beams. Typically, a confocal arrangement of lenses with a diaphragm in the focal plane is used for intracavity spatial filtering. Such conventional…

We demonstrate experimentally and support by a theoretical analysis an effect of asymmetric spectrum broadening, which results from doping of silver nanoparticles into a heavy-glass matrix, 90(0.5WO3-0.3SbPO4-0.2PbO)-10AgCl. The strong…

Optics · Physics 2015-05-27 N. Zhavoronkov , R. Driben , B. A. Bregadiolli , M. Nalin , B. A. Malomed

The combination of single photon emitters (quantum dots) and tailored metal nanoparticles with defined size and shape allows a detailed study of the interaction between light and matter. The enhanced optical near-field of the nanoparticles…

Mesoscale and Nanoscale Physics · Physics 2017-04-21 Gernot Schaffernak , Christian Gruber , Joachim R. Krenn , Markus Krug , Marija Gašparić , Martin Belitsch , Andreas Hohenau

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

The fluorescence of organic fluorophore molecules is enhanced when they are placed in contact with certain metals (Al, Ag, Cu, Au, etc.) whose surface plasmon waves couple into the radiative modes of the molecules and increase the radiative…

Mesoscale and Nanoscale Physics · Physics 2015-04-21 S. Sarkar , B. Kanchibotla , J. D. Nelson , J. D. Edwards , J. Anderson , G. C. Tepper , S. Bandyopadhyay