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Protein phosphorylation is a reversible post-translational modification commonly used by cell signaling networks to transmit information about the extracellular environment into intracellular organelles for the regulation of the activity…

Molecular Networks · Quantitative Biology 2016-11-17 Marylens Hernandez , Alexander Lachmann , Shan Zhao , Kunhong Xiao , Avi Ma'ayan

Proteins with multiple binding sites play important roles in cell signaling systems by nucleating protein complexes in which, for example, enzymes and substrates are co-localized. Proteins that specialize in this function are called by a…

Molecular Networks · Quantitative Biology 2011-09-12 Jin Yang , William S. Hlavacek

Multistationarity in biological systems is a mechanism of cellular decision making. In particular, signaling pathways regulated by protein phosphorylation display features that facilitate a variety of responses to different biological…

Subcellular Processes · Quantitative Biology 2011-07-05 Elisenda Feliu , Carsten Wiuf

Multisite phosphorylation networks are encountered in many intracellular processes like signal transduction, cell-cycle control or nuclear signal integration. In this contribution networks describing the phosphorylation and…

Molecular Networks · Quantitative Biology 2013-07-03 Katharina Holstein , Dietrich Flockerzi , Carsten Conradi

Rebinding kinetics of molecular ligands plays a critical role in biomachinery, from regulatory networks to protein transcription, and is also a key factor for designing drugs and high-precision biosensors.In this study, we investigate…

Biological Physics · Physics 2018-07-12 Aykut Erbas , Monica Olvera de la Cruz , John F. Marko

Reliably distinguishing between cells based on minute differences in receptor density is crucial for cell-cell or virus-cell recognition, the initiation of signal transduction and selective targeting in directed drug delivery. Such sharp…

Soft Condensed Matter · Physics 2021-10-04 Christine Linne , Daniele Visco , Stefano Angioletti-Uberti , Liedewij Laan , Daniela J. Kraft

Rebinding of dissociated ligands from cell surface proteins can confound quantitative measurements of dissociation rates important for characterizing the affinity of binding interactions. This can be true also for in vitro techniques such…

Quantitative Methods · Quantitative Biology 2007-05-23 Manoj Gopalakrishnan , Kimberly Forsten-Williams , Theressa R. Cassino , Luz Padro , Thomas E. Ryan , Uwe C. Tauber

Cells use surface receptors to estimate the concentration of external ligands. Limits on the accuracy of such estimations have been well studied for pairs of ligand and receptor species. However, the environment typically contains many…

Molecular Networks · Quantitative Biology 2015-06-02 Vijay Singh , Ilya Nemenman

Multivalent particles have the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency greatly increases the…

Soft Condensed Matter · Physics 2017-04-04 Tine Curk , Jure Dobnikar , Daan Frenkel

In this article, we study the kinetics of reversible ligand binding to receptors on a spherical cell surface using a self-consistent stochastic theory. Binding, dissociation, diffusion and rebinding of ligands are incorporated into the…

Subcellular Processes · Quantitative Biology 2009-11-13 Shivam Ghosh , Manoj Gopalakrishnan , Kimberly Forsten-Williams

Phosphorelays are a class of signaling mechanisms used by cells to respond to changes in their environment. Phosphorelays (of which two-component systems constitute a special case) are particularly abundant in prokaryotes and have been…

Molecular Networks · Quantitative Biology 2011-09-26 Michael Knudsen , Elisenda Feliu , Carsten Wiuf

Protein nanoclustering is a characteristic feature of their activated state and is essential for forming numerous subcellular structures. The formation of these nanoclusters is highly dependent on a series of post-translational…

Biological Physics · Physics 2025-11-13 Olivier Destaing , Bertrand Fourcade

Macromolecular crowding affects biophysical processes as diverse as diffusion, gene expression, cell growth, and senescence. Yet, there is no comprehensive understanding of how crowding affects reactions, particularly multivalent binding.…

Soft Condensed Matter · Physics 2024-01-15 Tomasz Skóra , Mathijs Janssen , Andreas Carlson , Svyatoslav Kondrat

The effects of ligand binding on protein structures and their in vivo functions carry numerous implications for modern biomedical research and biotechnology development efforts such as drug discovery. Although several deep learning (DL)…

Machine Learning · Computer Science 2026-03-24 Alex Morehead , Nabin Giri , Jian Liu , Pawan Neupane , Jianlin Cheng

The polarisation of cells and tissues is fundamental for tissue morphogenesis during biological development and regeneration. A deeper understanding of biological polarity pattern formation can be gained from the consideration of pattern…

Cell Behavior · Quantitative Biology 2017-10-23 Karl B. Hoffmann , Anja Voss-Böhme , Jochen C. Rink , Lutz Brusch

Ligand-receptor interactions are fundamental to many biological processes. For example in antibody-based immunotherapies, the dynamics of an antibody binding with its target antigen directly influence the potency and efficacy of monoclonal…

Other Quantitative Biology · Quantitative Biology 2025-01-17 Luke A Heirene , Helen M Byrne , James W T Yates , Eamonn A Gaffney

Positrons bind to molecules leading to vibrational excitation and spectacularly enhanced annihilation. Whilst positron binding energies have been measured via resonant annihilation spectra for $\sim$90 molecules in the past two decades, an…

Atomic Physics · Physics 2023-03-23 J. Hofierka , B. Cunningham , C. M. Rawlins , C. H. Patterson , D. G. Green

Multistationarity in molecular systems underlies switch-like responses in cellular decision making. Determining whether and when a system displays multistationarity is in general a difficult problem. In this work we completely determine the…

Molecular Networks · Quantitative Biology 2020-04-15 E. Feliu , N. Kaihnsa , T. de Wolff , O. Yürük

Binding of a ligand on a protein changes the flexibility of certain parts of the protein, which directly affects its function. These changes are not the same at each point, some parts become more flexible and some others become stiffer.…

Biomolecules · Quantitative Biology 2015-01-13 Burak Erman

Double phosphorylation of protein kinases is a common feature of signalling cascades. This motif may reduce cross-talk between signalling pathways, as the second phosphorylation site allows for proofreading, especially when phosphorylation…

Molecular Networks · Quantitative Biology 2015-05-26 Thomas E. Ouldridge , Pieter Rein ten Wolde