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Cytoskeletal networks of biopolymers are cross-linked by a variety of proteins. Experiments have shown that dynamic cross-linking with physiological linker proteins leads to complex stress relaxation and enables network flow at long times.…

We examine the mechanism of bundling of cytoskeletal actin filaments by two representative bundling proteins, fascin and espin. Small-angle X-ray studies show that increased binding from linkers drives a systematic \textit{overtwist} of…

Soft Condensed Matter · Physics 2015-05-14 Homin Shin , Kirstin R. Purdy Drew , James R. Bartles , Gerard C. L. Wong , Gregory M. Grason

The mechanical properties of cells are dominated by the cytoskeleton, an interconnected network of long elastic filaments. The connections between the filaments are provided by crosslinking proteins, which constitute, next to the filaments,…

Soft Condensed Matter · Physics 2012-10-09 Claus Heussinger

Cells make use of semi-flexible biopolymers such as actin or intermediate filaments to control their local viscoelastic response by dynamically adjusting the concentration and type of cross-linker molecules. The microstructure of the…

Soft Condensed Matter · Physics 2009-11-13 Yuxia Luan , Oliver Lieleg , Bernd Wagner , Andreas R. Bausch

While actin bundles are used by living cells for structural fortification, the microscopic origin of the elasticity of bundled networks is not understood. Here, we show that above a critical concentration of the actin binding protein…

Soft Condensed Matter · Physics 2007-08-30 O. Lieleg , M. M. A. E. Claessens , C. Heussinger , E. Frey , A. R. Bausch

The synthetic actin network demands great interest as bio-material due to its soft and wet nature that mimic many biological scaffolding structures. The study of these structures can contribute to a better understanding of this new…

Soft Condensed Matter · Physics 2017-02-10 Horacio López-Menéndez , José Félix Rodríguez

Actin is one of the most studied cytoskeleton proteins showing a very rich span of structures. It can self-assemble actively into dynamical structures that govern the mechanical properties of the cell, its motility and its division.…

Soft Condensed Matter · Physics 2020-03-17 Maayan Levin , Raya Sorkin , David Pine , Rony Granek , Anne Bernheim-Groswasser , Yael Roichman

The synthetic actin network arouses great interest as bio-material due to its soft and wet nature that mimics many biological scaffolding structures. Inside the cell, the actin network is regulated by tens of actin-binding proteins (ABP's),…

Soft Condensed Matter · Physics 2019-03-19 Horacio Lopez-Menendez

We construct a coarse-grained model of parallel actin bundles crosslinked by compact, globular bundling proteins, such as fascin and espin, necessary components of filapodial and mechanosensory bundles. Consistent with structural…

Soft Condensed Matter · Physics 2013-05-29 Homin Shin , Gregory M. Grason

Combining simulations and theory I study the interplay between bundle elastic degrees of freedom and crosslink binding propensity. By slowly driving bundles into a deformed configuration, and depending on the mechanical stiffness of the…

Soft Condensed Matter · Physics 2011-05-20 Claus Heussinger

The cytoskeleton protein actin assembles into large bundles when supporting stresses in the cell, but grows into a fine branched network to induce cell motion. Such self-organization processes are studied in artificial networks of…

Soft Condensed Matter · Physics 2018-06-13 Adar Sonn-Segev , Anne Bernheim-Groswasser , Yael Roichman

The formation of bundles composed of actin filaments and cross-linking proteins is an essential process in the maintenance of the cells' cytoskeleton. It has also been recreated by in-vitro experiments, where actin networks are routinely…

Soft Condensed Matter · Physics 2009-11-13 Nir S. Gov

The actin cytoskeleton in living cells has many types of crosslinkers. The mechanical interplay between these different crosslinker types is an open issue in cytoskeletal mechanics. We develop a framework to study the cooperativity and…

Soft Condensed Matter · Physics 2015-05-28 Moumita Das , D. A. Quint , J. M. Schwarz

The semiflexible F-actin network of the cytoskeleton is cross-linked by a variety of proteins including filamin, which contain Ig-domains that unfold under applied tension. We examine a simple semiflexible network model cross-linked by such…

Soft Condensed Matter · Physics 2009-11-11 B. A. DiDonna , Alex J. Levine

The phase behavior of charged rods in the presence of inter-rod linkers is studied theoretically as a model for the equilibrium behavior underlying the organization of actin filaments by linker proteins in the cytoskeleton. The presence of…

Soft Condensed Matter · Physics 2016-08-31 Itamar Borukhov , Robijn F. Bruinsma , William M. Gelbart , Andrea J. Liu

Subcellular protein complexes and organelles exhibit diverse dynamic behaviors that reflect the mechanical constraints and organization of the intracellular environment. Although some structures follow classical Brownian motion, many…

Quantifying the influence of microscopic details on the dynamics of development of the overall structure of a filamentous network is important in a number of biologically relevant contexts, but it is not obvious what order parameters can be…

Biological Physics · Physics 2021-01-04 Yossi Eliaz , Francois Nedelec , Greg Morrison , Herbert Levine , Margaret S. Cheung

While most chemical bonds weaken under the action of mechanical force (called slip bond behavior), nature has developed bonds that do the opposite: their lifetime increases as force is applied. While such catch bonds have been studied quite…

Soft Condensed Matter · Physics 2023-03-29 José Ruiz-Franco , Justin Tauber , Jasper van der Gucht

The actin cytoskeleton is composed of multiple networks which are specialized for several processes such as cell motility or cell division. Each of these networks are composed of organized actin microfilaments which are decorated with…

Biological Physics · Physics 2018-11-26 Thomas Le Goff , Alphée Michelot

We report on the nonlinear mechanical properties of a statistically homogeneous, isotropic semiflexible network cross-linked by polymers containing numerous small unfolding domains, such as the ubiquitous F-actin cross-linker Filamin. We…

Soft Condensed Matter · Physics 2013-05-29 B. A. DiDonna , Alex J. Levine
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