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The spatio-temporal organization of proteins within the cell membrane can affect numerous biological functions, including cell signaling, communication, and transportation. Deviations from normal spatial arrangements have been observed in…

Biomolecules · Quantitative Biology 2023-10-25 Yasaman Moradi , Jerry SH Lee , Andrea M. Armani

We demonstrate the stimulated Raman near-field microscopy of few molecules, measured only using near-field optical forces thereby eliminating the need for far-field optical detection. The molecules were excited in the near-field without…

We investigated the localized electronic properties of nanoporous gold films by using an ultra-high vacuum scanning tunneling microscope at low temperature (4.2 K). Second derivative scanning tunneling spectroscopy shows the plasmon peaks…

Materials Science · Physics 2014-06-10 H. W. Liu , R. Nishitani , T. Fujita , W. Li , L. Zhang , X. Y. Lang , P. Richard , K. S. Nakayama , X. Chen , M. W. Chen , Q. K. Xue

Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near-fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the…

A scanning tunneling microscope (STM) can do more than atomic imaging and manipulation. Its tunneling current can also be used for the excitation of light, converting electron energy to photon energy. STM based single-molecule…

Optics · Physics 2019-01-01 Yao Zhang , Yang Zhang , Zhenchao Dong

Infrared spectroscopy is the technique of choice for chemical identification of biomolecules through their vibrational fingerprints. However, infrared light interacts poorly with nanometric size molecules. Here, we exploit the unique…

There is a great interest in the scientific community to perform calorimetry on samples having mass in the nanogram range. A detailed knowledge of the energy (heat) exchange in the fast growing family of micro- and nano-systems could…

Applied Physics · Physics 2021-12-02 S. Veronesi , T. Papa , Y. Murata , S. Heun

Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is…

Genomics · Quantitative Biology 2007-07-02 Mireille Chevallet , Sylvie Luche , Thierry Rabilloud

It has been a general trend to develop low-voltage electron microscopes due to their high imaging contrast of the sample and low radiation damage. Atom-resolved transmission electron microscopes with voltages as low as 15-40 kV have been…

Instrumentation and Detectors · Physics 2015-12-29 Wei-Tse Chang , Chun-Yueh Lin , Wei-Hao Hsu , Mu-Tung Chang , Yi-Sheng Chen , En-Te Hwu , Ing-Shouh Hwang

Transmission electron microscopy (TEM) is a powerful imaging tool that has found broad application in materials science, nanoscience and biology(1-3). With the introduction of aberration-corrected electron lenses, both the spatial…

We report on the fabrication of two-dimensional periodic photonic nanostructures by nanoimprint lithography and dry etching, and their integration into a 1-{\mu}m-thin mono-crystalline silicon solar cell. Thanks to the periodic…

Mesoscale and Nanoscale Physics · Physics 2012-12-17 Christos Trompoukis , Ounsi El Daif , Valérie Depauw , Ivan Gordon , Jef Poortmans

The polymer of intrinsic microporosity PIM 1 is employed to disperse and deposit a Pt at titanate nanosheet photocatalyst film. The resulting microporous films allow electrolyte and oxygen permeation to give conventional oxygen reduction…

Melting and hybridization of DNA-capped gold nanoparticle networks are investigated with optical absorption spectroscopy. Single-stranded, 12-base DNA-capped gold nanoparticles are linked with complementary, single-stranded, 24-base linker…

Biological Physics · Physics 2009-11-07 Ching-Hwa Kiang

Current methods for producing single-walled carbon nanotubes (SWNTs) lead to heterogeneous samples containing mixtures of metallic and semiconducting species with a variety of lengths and defects. Optical detection at the single nanotube…

Large-area chemically synthesized single-crystal metals with nanometer-scale thickness have emerged as promising materials for on-chip nanophotonic applications, owing to their superior plasmonic properties compared to nanofabricated…

A quantitative single-molecule tip-enhanced Raman spectroscopy (TERS) study at room temperature remained a challenge due to the rapid structural dynamics of molecules exposed to air. Here, we demonstrate the hyperspectral TERS imaging of…

Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterisation of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background…

Single-atom-resolved detection in optical lattices using quantum-gas microscopes has enabled a new generation of experiments in the field of quantum simulation. Fluorescence imaging of individual atoms has so far been achieved for bosonic…

Single-pixel imaging, originally developed in light optics, facilitates fast three-dimensional sample reconstruction, as well as probing with light wavelengths undetectable by conventional multi-pixel detectors. However, the spatial…

Introduced more than fifty years ago, dynamic light scattering is routinely used to determine the size distribution of colloidal suspensions, as well as of macromolecules in solution, such as proteins, nucleic acids, and their complexes.…