Understanding the Methyl-TROSY effect over a wide range of magnetic fields
Abstract
The use of relaxation interference in the methyl Transverse Relaxation-Optimized SpectroscopY (TROSY) experiment has opened new avenues for the study of large proteins and protein assemblies in nuclear magnetic resonance. So far, the theoretical description of the methyl-TROSY experiment has been limited to the slow-tumbling approximation, which is correct for large proteins on high field spectrometers. In a recent paper, favorable relaxation interference was observed in the methyl groups of a small protein at a magnetic field as low as 0.33 T, well outside the slow-tumbling regime. Here, we present a model to describe relaxation interference in methyl groups over a broad range of magnetic fields, not limited to the slow-tumbling regime. We predict that the type of multiple-quantum transitions that show favorable relaxation properties change with the magnetic field. Under the condition of fast methyl-group rotation, methyl-TROSY experiments can be recorded over the entire range of magnetic fields from a fraction of 1 T up to 100 T.
Keywords
Cite
@article{arxiv.1903.04452,
title = {Understanding the Methyl-TROSY effect over a wide range of magnetic fields},
author = {Nicolas Bolik-Coulon and Samuel F. Cousin and Pavel Kadeřávek and Jean-Nicolas Dumez and Fabien Ferrage},
journal= {arXiv preprint arXiv:1903.04452},
year = {2019}
}