The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods
Abstract
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, such as primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
Cite
@article{arxiv.2412.12965,
title = {The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods},
author = {Ziv Yaniv and Ifeanyichukwu U. Anidi and Leanne Arakkal and Armando J. Arroyo-Mejías and Rebecca T. Beuschel and Katy Börner and Colin J. Chu and Beatrice Clark and Menna R. Clatworthy and Jake Colautti and Fabian Coscia and Joshua Croteau and Saven Denha and Rose Dever and Walderez O. Dutra and Sonja Fritzsche and Spencer Fullam and Michael Y. Gerner and Anita Gola and Kenneth J. Gollob and Jonathan M. Hernandez and Jyh Liang Hor and Hiroshi Ichise and Zhixin Jing and Danny Jonigk and Evelyn Kandov and Wolfgang Kastenmüller and Joshua F. E. Koenig and Aanandita Kothurkar and Rosa K. Kortekaas and Alexandra Y. Kreins and Ian T. Lamborn and Yuri Lin and Katia Luciano Pereira Morais and Aleksandra Lunich and Jean C. S. Luz and Ryan B. MacDonald and Chen Makranz and Vivien I. Maltez and John E. McDonough and Ryan V. Moriarty and Juan M. Ocampo-Godinez and Vitoria M. Olyntho and Annette Oxenius and Kartika Padhan and Kirsten Remmert and Nathan Richoz and Edward C. Schrom and Wanjing Shang and Lihong Shi and Rochelle M. Shih and Emily Speranza and Salome Stierli and Sarah A. Teichmann and Tibor Z. Veres and Megan Vierhout and Brianna T. Wachter and Adam K. Wade-Vallance and Margaret Williams and Nathan Zangger and Ronald N. Germain and Andrea J. Radtke},
journal= {arXiv preprint arXiv:2412.12965},
year = {2025}
}