T4 DNA condensation in water-alcohol media
Soft Condensed Matter
2011-07-22 v1
Abstract
The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.
Cite
@article{arxiv.1107.4215,
title = {T4 DNA condensation in water-alcohol media},
author = {M. O. Gallyamov and O. A. Pyshkina and V. G. Sergeyev and I. V. Yaminsky},
journal= {arXiv preprint arXiv:1107.4215},
year = {2011}
}
Comments
in Russian