Silver Staining of 2D Electrophoresis Gels

Thierry Rabilloud

doi:10.1007/978-1-61779-885-6_5

Silver Staining of 2D Electrophoresis Gels

Genomics 2012-06-15 v1

Authors: Thierry Rabilloud

View on arXiv ↗ PDF ↗ DOI ↗

Abstract

Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed.

Keywords

silver staining two-dimensional electrophoresis protein detection mass spectrometry compatibility quantitative response optimized protocol

Cite

@article{arxiv.1206.3004,
  title  = {Silver Staining of 2D Electrophoresis Gels},
  author = {Thierry Rabilloud},
  journal= {arXiv preprint arXiv:1206.3004},
  year   = {2012}
}

Comments

arXiv admin note: substantial text overlap with arXiv:0904.3535

Related papers

View all related →