Sensor-Based Turbidostat Operation Enables Biomass Setpoint Regulation and Productivity Improvement in semi-industrial Microalgae Raceway Pond

This work presents the experimental validation of a turbidostat strategy for biomass control in a semi-industrial outdoor raceway reactor. The proposed approach regulates biomass concentration by automatically triggering dilution when the online biomass estimate exceeds a predefined threshold. To ensure safe outdoor operation, dilution was restricted to daylight periods, avoiding biomass removal under low-radiation conditions. The strategy was implemented through an industrial control architecture using an optical monitoring system for online biomass estimation. Experiments were conducted over 14 consecutive days in an 80 m$^2$ (12000 L) raceway reactor. A second parallel reactor operated in chemostat mode, with a nominal dilution of 20 % of the total volume during operating days, provided contextual information under the same outdoor conditions. The analysis focuses on the ability of the sensor-based strategy to configure and maintain the desired biomass concentration, rather than on a direct reactor-to-reactor performance ranking. During the campaign, the biomass threshold in the turbidostat reactor was changed from 1.0 to 0.8 g L$^{-1}$, demonstrating the flexibility enabled by online biomass monitoring. Excluding initial adjustment and transition days, harvested areal productivity increased from 9.52 to 23.20 g m$^{-2}$ d$^{-1}$ after reducing the operating threshold. The overall biomass balance also showed higher net areal productivity in the turbidostat reactor, reaching 20.34 g m$^{-2}$ d$^{-1}$ compared with 11.16 g m$^{-2}$ d$^{-1}$ in the parallel chemostat reactor. These results demonstrate the feasibility of robust turbidostat-based biomass control in large-scale outdoor raceway photobioreactors.