rSHG: Re-scan Second Harmonic Generation Microscopy
Abstract
Second Harmonic Generation Microscopy (SHG) is generally acknowledged as a powerful tool for the label-free 3D visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Although the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we quantitatively show on collagenous tissues that by combining SHG imaging with re-scan microscopy resolutions that surpass the diffraction limit with ~1.4x become available. Besides Re-scan Second Harmonic Generation Microscopy (rSHG), we demonstrate as well super-resolved Re-scan Two-Photon Excited Fluorescence Microscopy (rTPEF). These two techniques are implemented by modifying a Re-scan Confocal Microscope (RCM), retaining its initial function, resulting thus in a multimodal rSHG/rTPEF/RCM system. Given the simplicity and flexibility of re-scan microscopy, we consider that the reported results are likely to augment the number and nature of applications relying on super-resolved non-linear optical imaging.
Cite
@article{arxiv.2207.02002,
title = {rSHG: Re-scan Second Harmonic Generation Microscopy},
author = {Stefan G. Stanciu and Radu Hristu and George A. Stanciu and Denis E. Tranca and Lucian Eftimie and Adrian Dumitru and Mariana Costache and Harald A. Stenmark and Harm Manders and Amit Cherian and Mariliis Tark-Dame and Erik M. M. Manders},
journal= {arXiv preprint arXiv:2207.02002},
year = {2022}
}