Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme

Gergely Katona; Philippe Carpentier; Vincent Nivière; Patricia Amara; Virgile Adam; Jérémy Ohana; Nikolay Tsanov; Dominique Bourgeois

Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme

Biomolecules 2014-12-17 v1

Authors: Gergely Katona , Philippe Carpentier , Vincent Nivière , Patricia Amara , Virgile Adam , Jérémy Ohana , Nikolay Tsanov , Dominique Bourgeois

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Abstract

Iron-peroxide intermediates are central in the reaction cycle of many iron-containing biomolecules. We trapped iron(III)-(hydro)peroxo species in crystals of superoxide reductase (SOR), a nonheme mononuclear iron enzyme that scavenges superoxide radicals. X-ray diffraction data at 1.95 angstrom resolution and Raman spectra recorded in crystallo revealed iron-(hydro)peroxo intermediates with the (hydro)peroxo group bound end-on. The dynamic SOR active site promotes the formation of transient hydrogen bond networks, which presumably assist the cleavage of the iron-oxygen bond in order to release the reaction product, hydrogen peroxide.

Keywords

Raman-assisted crystallography nonheme iron enzyme superoxide reductase iron-peroxide intermediates end-on binding X-ray diffraction hydrogen peroxide

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@article{arxiv.1412.5040,
  title  = {Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme},
  author = {Gergely Katona and Philippe Carpentier and Vincent Nivière and Patricia Amara and Virgile Adam and Jérémy Ohana and Nikolay Tsanov and Dominique Bourgeois},
  journal= {arXiv preprint arXiv:1412.5040},
  year   = {2014}
}

Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme

Abstract

Keywords

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