Parameter estimation in FACS-seq enables high-throughput characterization of phenotypic heterogeneity
Quantitative Methods
2020-10-08 v1
Abstract
Phenotypic heterogeneity is a most fascinating property of a population of cells, which shows the differences among individuals even with the same genetic background and extracellular environmental conditions. However, the lack of high-throughput analysis of phenotypic diversity has limited our research progress. To deal with it, we constructed a novel parameter estimation method in FACS-seq, a commonly used experimental framework, to achieve simultaneous characterization of thousands of variants in a library. We further demonstrated the model's ability in estimating the expression properties of each variant, which we believe can help to decipher the mechanisms of phenotypic heterogeneity.
Cite
@article{arxiv.2010.03317,
title = {Parameter estimation in FACS-seq enables high-throughput characterization of phenotypic heterogeneity},
author = {Huibao Feng and Chong Zhang},
journal= {arXiv preprint arXiv:2010.03317},
year = {2020}
}