We investigated the ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) in Ewing sarcoma cells, in the perspective of in vivo anti-cancer nucleic acid drug delivery. siRNA was adsorbed onto NDs previously coated with cationic polymer. Cell uptake of NDs has been demonstrated by taking advantage of NDs intrinsic fluorescence coming from embedded color center defects. Cell toxicity of these coated NDs was shown to be low. Consistent with the internalization efficacy, we have shown a specific inhibition of EWS/Fli-1 gene expression at the mRNA and protein level by the ND vectorized siRNA in a serum containing medium.
@article{arxiv.1106.2252,
title = {Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells},
author = {Anna Alhaddad and Marie-Pierre Adam and Jacques Botsoa and Géraldine Dantelle and Sandrine Perruchas and Thierry Gacoin and Christelle Mansuy and Solange Lavielle and Claude Malvy and François Treussart and Jean-Rémi Bertrand},
journal= {arXiv preprint arXiv:1106.2252},
year = {2011}
}