Multiwavelength Observations of the Apparently Non-repeating FRB 20250316A

The physical origin of fast radio bursts (FRBs) remains uncertain. Although multiwavelength observations have been widely conducted, only Galactic FRB~20200428D is associated with an X-ray burst from the magnetar SGR J1935+2154. Here, we present multiwavelength follow-up observations of the nearby bright FRB~20250316A, including the Five-hundred-meter Aperture Spherical radio Telescope (FAST), Einstein Probe (EP) X-ray mission, Chandra X-ray Observatory, Wide Field Survey Telescope (WFST) and Space Variable Object Monitor/Visible Telescope (SVOM/VT). The 13.08-hour FAST follow-up campaign without pulse detection requires an energy distribution flatter than those of well-known repeating FRBs, suggesting that this burst is likely a one-off event. A prompt EP follow-up and multi-epoch observational campaign totaling $>$ 100 ks led to the detection of an X-ray source within the angular resolution of its Follow-up X-ray Telescope (FXT, $10^{\prime\prime}$). A subsequent Chandra observation revealed this source to be offset by $7^{\prime\prime}$ from the FRB position, and established a 0.5-10 keV flux upper limit of $7.6\times 10^{-15}$ $\rm erg\,cm^{-2}\,s^{-1}$ at the FRB position, corresponding to $\sim 10^{39}$ $\rm erg\,s^{-1}$ at the 40 Mpc distance of the host galaxy NGC~4141. These results set one of the most stringent limits on X-ray emission from a non-repeating FRB, disfavoring ultra-luminous X-ray sources (ULXs) as counterparts of apparently one-off FRBs and offering critical insights into afterglow models. Our study suggests that an arcsecond localization of both the FRB and its potential X-ray counterpart is essential for exploring the X-ray counterpart of an FRB.