We introduce the mixed-norm amalgam spaces (Lp,Ls)(Rn) and (Lp,Ls)α(Rn), and show their some basic properties. In addition, we find the predual H(p′,s′,α′) of mixed-norm amalgam spaces (Lp,ℓs)α(Rn) by the dual spaces (Lp′,ℓs′)(Rn) of (Lp,ℓs)(Rn), where (Lp,Ls)(Rn)=(Lp,ℓs)(Rn) and (Lp,Ls)α(Rn)=(Lp,ℓs)α(Rn). Then, we study the strong-type estimates for fractional integral operators Iγ on mixed-norm amalgam spaces (Lp,Ls)α(Rn). And, the strong-type estimates of linear commutators [b,Iγ] generated by b∈BMO(Rn) and Iγ on mixed-norm amalgam spaces (Lp,Ls)α(Rn) are established as well. Furthermore, based on the dual theorem, the characterization of BMO(Rn) by the boundedness of [b,Iγ] from (Lp,Ls)α(Rn) to (Lq,Ls)β(Rn) is given, which is a new result even for the classical amalgam spaces.