Head-mounted miniaturized microscopes, commonly known as miniscopes, have undergone rapid development and seen widespread adoption over the past two decades, enabling the imaging of neural activity in freely-behaving animals such as rodents, songbirds, and non-human primates. These miniscopes facilitate numerous studies that are not feasible with head-fixed preparations. Recent advancements have enhanced their capabilities, allowing for faster imaging, larger fields of view, and deeper brain penetration. In this review, we examine the latest progress in one-photon and multi-photon miniscopes. We highlight the unique opportunities these devices present for neuroscience research, discuss the current technical challenges, and explore emerging technologies that promise to advance the development of miniscopes.
@article{arxiv.2603.11435,
title = {Miniaturized microscopes to study neural dynamics in freely-behaving animals},
author = {Weijian Zong and Weijian Yang},
journal= {arXiv preprint arXiv:2603.11435},
year = {2026}
}