Long-read sequencing (LRS) is able to simultaneously capture information about both DNA sequence and modifications, such as CpG methylation in a single sequencing experiment. Here we present Methylation Operation Wizard (MeOW), a program to identify and prioritize differentially methylated regions (DMRs) genome-wide using LRS data. MeOW can be run using either a file containing counts of per-nucleotide methylated CpG sites or with a bam file containing modified base tags.
@article{arxiv.2402.17182,
title = {Methylation Operation Wizard (MeOW): Identification of differentially methylated regions in long-read sequencing data},
author = {Miranda PG Zalusky and Danny E Miller},
journal= {arXiv preprint arXiv:2402.17182},
year = {2024}
}