Kinetics and thermodynamics of DNA polymerases with exonuclease proofreading
Subcellular Processes
2018-01-04 v1 Biological Physics
Abstract
Kinetic theory and thermodynamics are applied to DNA polymerases with exonuclease activity, taking into account the dependence of the rates on the previously incorportated nucleotide. The replication fidelity is shown to increase significantly thanks to this dependence at the basis of the mechanism of exonuclease proofreading. In particular, this dependence can provide up to a hundred-fold lowering of the error probability under physiological conditions. Theory is compared with numerical simulations for the DNA polymerases of T7 viruses and human mitochondria.
Keywords
Cite
@article{arxiv.1604.02554,
title = {Kinetics and thermodynamics of DNA polymerases with exonuclease proofreading},
author = {Pierre Gaspard},
journal= {arXiv preprint arXiv:1604.02554},
year = {2018}
}
Comments
Physical Review E (2016)