Integrative omics framework for characterization of coral reef ecosystems from the Tara Pacific expedition
Abstract
Coral reef science is a fast-growing field propelled by the need to better understand coral health and resilience to devise strategies to slow reef loss resulting from environmental stresses. Key to coral resilience are the symbiotic interactions established within a complex holobiont, i.e. the multipartite assemblages comprising the host coral organism, endosymbiotic dinoflagellates, bacteria, archaea, fungi, and viruses. Tara Pacific is an ambitious project built upon the experience of previous Tara Oceans expeditions, and leveraging state-of-the-art sequencing technologies and analyses to dissect the biodiversity and biocomplexity of the coral holobiont screened across most archipelagos spread throughout the entire Pacific Ocean. Here we detail the Tara Pacific workflow for multi-omics data generation, from sample handling to nucleotide sequence data generation and deposition. This unique multidimensional framework also includes a large amount of concomitant metadata collected side-by-side that provide new assessments of coral reef biodiversity including micro-biodiversity and shape future investigations of coral reef dynamics and their fate in the Anthropocene.
Cite
@article{arxiv.2207.02475,
title = {Integrative omics framework for characterization of coral reef ecosystems from the Tara Pacific expedition},
author = {Caroline Belser and Julie Poulain and Karine Labadie and Frederick Gavory and Adriana Alberti and Julie Guy and Quentin Carradec and Corinne Cruaud and Corinne da Silva and Stefan Engelen and Paul Mielle and Aude Perdereau and Gaelle Samson and Shahinaz Gaz and Genoscope Technical Team and Christian R Voolstra and Pierre E Galand and J. Michel Flores and Benjamin Cc Hume and Gabriela Perna and Maren Ziegler and Hans-Joachim Ruscheweyh and Emilie Boissin and Sarah Romac and Guillaume Bourdin and Guillaume Iwankow and Clémentine Moulin and David A Paz García and Tara Pacific Consortium Coordinators and Claude Scarpelli and E Krame Jacoby and Pedro H Oliveira and Jean-Marc Aury and Denis Allemand and Serge Planes and Patrick Wincker},
journal= {arXiv preprint arXiv:2207.02475},
year = {2022}
}