High-throughput screening of encapsulated islets using wide-field lens-free on-chip imaging
Abstract
Islet microencapsulation is a promising solution to diabetes treatment, but its quality control based on manual microscopic inspection is extremely low-throughput, highly variable and laborious. This study presents a high-throughput islet-encapsulation quality screening system based on lens-free on-chip imaging with a wide field-of-view of 18.15 cm^2, which is more than 100 times larger than that of a lens-based optical microscope, enabling it to image and analyze ~8,000 microcapsules in a single frame. Custom-written image reconstruction and processing software provides the user with clinically important information, such as microcapsule count, size, intactness, and information on whether each capsule contains an islet. This high-throughput and cost-effective platform can be useful for researchers to develop better encapsulation protocols as well as perform quality control prior to transplantation.
Keywords
Cite
@article{arxiv.1803.03534,
title = {High-throughput screening of encapsulated islets using wide-field lens-free on-chip imaging},
author = {Yibo Zhang and Michael Alexander and Sam Yang and Yinxu Bian and Elliot Botvinick and Jonathan R. T. Lakey and Aydogan Ozcan},
journal= {arXiv preprint arXiv:1803.03534},
year = {2018}
}