Graphene-induced energy transfer (GIET) is a recently developed fluorescence-spectroscopic technique that achieves sub-nanometric optical localization of fluorophores along the optical axis of a microscope. GIET is based on the near-field energy transfer from an optically excited fluorescent molecule to a single sheet of graphene. It has been successfully used for estimating inter-leaflet distances of single lipid bilayers, and for investigating the membrane organization of living mitochondria. In this study, we use GIET to measure the cholesterol-induced subtle changes of membrane thickness at the nanoscale. We quantify membrane thickness variations in supported lipid bilayers (SLBs) as a function of lipid composition and increasing cholesterol content. Our findings demonstrate that GIET is an extremely sensitive tool for investigating nanometric structural changes in bio-membranes.
@article{arxiv.2211.03517,
title = {Graphene-Induced Energy Transfer for Quantitative Membrane Biophysics at Sub-Nanometer Resolution},
author = {Tao Chen and Arindam Ghosh and Jörg Enderlein},
journal= {arXiv preprint arXiv:2211.03517},
year = {2022}
}