Global SAXS data analysis for multilamellar vesicles: Evolution of the scattering density profile (SDP) model
Abstract
We adapted the highly successful scattering density profile (SDP) model, which is used to jointly analyze small angle x-ray and neutron scattering data from unilamellar vesicles, for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). By using a genetic algorithm, this new method is capable of providing high resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone x-ray data. Important structural parameters, such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. Results are in good agreement with previously reported SDP data, which used both neutron and x-ray data. The addition of deuterated and non-deuterated MLV neutron data to the analysis improved lipid backbone information, but did not improve, within experimental uncertainties, the structural information regarding bilayer thickness and area per lipid.
Cite
@article{arxiv.1309.5237,
title = {Global SAXS data analysis for multilamellar vesicles: Evolution of the scattering density profile (SDP) model},
author = {Peter Heftberger and Benjamin Kollmitzer and Frederick A. Heberle and Jianjun Pan and Michael Rappolt and Heinz Amenitsch and Norbert Kucerka and John Katsaras and Georg Pabst},
journal= {arXiv preprint arXiv:1309.5237},
year = {2016}
}