We have developed a new model to describe FRET efficiency (E FRET) between freely-diffusing membrane probes in phase-separated bilayers (Finite Phase-separation FRET, or FP-FRET), that in principle applies to any system where phase domain dimensions are larger than ~Ro . Here we use Monte Carlo techniques to simulate E FRET for a range of probe partitioning behaviors and domain sizes, and then fit the simulated data to the FP-FRET model to recover simulation parameters. We find that FP-FRET can determine domain size to within 5% of simulated values for domain diameters up to ~ 5Ro, and to within 15% for diameters up to ~ 20Ro . We also investigated the performance of the model in cases where specific model assumptions are not valid.
@article{arxiv.1105.6254,
title = {Finite Phase-separation FRET II: Determination of domain size from simulated data},
author = {Frederick A. Heberle and Gerald W. Feigenson},
journal= {arXiv preprint arXiv:1105.6254},
year = {2011}
}