Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals
Biological Physics
2018-02-20 v3
Abstract
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray-scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. This approach provides a route to study the weak diffuse or lattice-transform signal arising from small crystals. The high speed of the particles is particularly well suited to upcoming MHz-repetition-rate x-ray free-electron lasers.
Keywords
Cite
@article{arxiv.1702.04014,
title = {Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals},
author = {Salah Awel and Richard A. Kirian and Max O. Wiedorn and Kenneth R. Beyerlein and Nils Roth and Daniel A. Horke and Dominik Oberthür and Juraj Knoska and Valerio Mariani and Andrew Morgan and Luigi Adriano and Alexandra Tolstikova and P. Lourdu Xavier and Oleksandr Yefanov and Andrew Aquila and Anton Barty and Shatabdi Roy-Chowdhury and Mark S. Hunter and Daniel James and Joseph S. Robinson and Uwe Weierstall and Andrei V. Rode and Saša Bajt and Jochen Küpper and Henry N. Chapman},
journal= {arXiv preprint arXiv:1702.04014},
year = {2018}
}