Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy
Abstract
Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.
Keywords
Cite
@article{arxiv.0708.1849,
title = {Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy},
author = {Ching-Ting Lin and En-Kuang Tien and Szu-Yuan Lee and Long-Sheng Lu and Chau-Chung Wu and Chen-Yuan Dong and Chii-Wann Lin},
journal= {arXiv preprint arXiv:0708.1849},
year = {2007}
}
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Submitted on behalf of TIMA Editions (http://irevues.inist.fr/tima-editions)