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Determining the Refractive Index of Human Hemoglobin Solutions by Kramers-Kronig Relations with an Improved Absorption Model

Biological Physics 2017-11-08 v1 Medical Physics Optics

Abstract

The real part of the refractive index (RI) of aqueous solutions of human hemoglobin is computed from their absorption spectra in the wavelength range 250nm1100nm250\,{\rm nm} - 1100\,{\rm nm} using the Kramers-Kronig (KK) relations and the corresponding uncertainty analysis is provided. The strong ultraviolet (UV) and infrared absorbance of the water outside this spectral range were taken into account in a previous study employing KK relations. We improve these results by including the concentration dependence of the water absorbance as well as by modeling the deep UV absorbance of hemoglobin's peptide backbone. The two free parameters of the model for the deep UV absorbance are fixed by a global fit.

Keywords

Cite

@article{arxiv.1711.02175,
  title  = {Determining the Refractive Index of Human Hemoglobin Solutions by Kramers-Kronig Relations with an Improved Absorption Model},
  author = {Jonas Gienger and Hermann Groß and Jörg Neukammer and Markus Bär},
  journal= {arXiv preprint arXiv:1711.02175},
  year   = {2017}
}

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